In Ouchterlony-type immunodiffusion gels, the cholera toxin produced by the classical Vibrio cholerae strain 569B was indistinguishable from the cholera toxin of the Eltor strain RJ1. However, the cholera toxin produced by both of these strains was incompletely cross-reactive with the cholera toxin produced by the Eltor strain 3083-2. The allele of the gene responsible for the 569B and RJ1 type of toxin was designated vct-1, and the allele responsible for the 3083-2 type of toxin was designated vct-2. The vct-J allele was transferred from RJ1 donors to 3083-2 recipients by conjugation. The vct locus was found to be between met and trp on the V. cholerae linkage map.
The results of a genetic cross between a
Vibrio cholerae
RJ1 donor and a
V. cholerae
3083-2 recipient suggest that the map position of
tox-1000
is between
his
and
trp
.
Chemical weed control has been widely adopted and has led to increased efficiency and reduced crop production costs. With the increased use of herbicides and the introduction of herbicide‐tolerant crops we have also seen an increase in herbicide resistant weeds which presents a challenge for farmers and land managers. It is incumbent upon the agriculture industry to be an indispensable partner in leading policy, research, education, and best management practices related to herbicide resistance. Corteva Agriscience is an active, engaged partner in herbicide resistance research, education, and communication globally to enable the long‐term sustainable use of herbicide‐tolerant crop traits and herbicides. Some of the key components of our commitment are highlighted in this Perspective paper and include memberships, partnerships, close involvement with CropLife International (and regional CropLife organizations), and Herbicide Resistance Action Committees at the Global, regional and country level, technical leadership and engagement in multiple scientific societies, and collaboration with universities and research institutes. Corteva is committed to advancing sustainable agriculture to enrich lives and our planet for generations to come and this drives our action through the entire product lifecycle and with our customers and consumers.
An enterotoxin A subunit-specific radioactive probe was used to correlate expression of vct-1 or vct-2 with the presence of specific restriction fragments in the DNAs of several wild-type and recombinant Vibrio cholerae strains. The data are consistent with the conclusion that vct is a cholera toxin structural gene. We previously reported that the gene vct specifies the antigenic structure of cholera toxin (CT) and is located between met and trp on the Vibrio cholerae genetic map (14). The vet-I
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.