Model experiments on rats with endotoxic shock induced by intraperitoneal injection of LPS Salmonella Typhi strain ty-4441 (20 mg/kg) showed that crossing of the vagus nerve innervating the spleen increased HR, stimulated production of antibodies, and moderated serotonergic activity of splenocytes. Pharmacological correction of the shock with muscarinic receptor antagonist atropine and its combinations with anticholinesterase agent galantamine or muscarinic and nicotinic cholinoreceptor agonist choline alfoscerate 30 min before shock modeling moderated HR and normalized B cell functions and serotonin level in the spleen.
Antimuscarinic effects of ipratropium bromide and atropine are associated with prevention of the development of Arthus reaction, while the cytostatic effects of cyclophosphamide and doxorubicin lead to involution of the thymus and spleen, suppression of antibody production, and aggravation of inflammation, which causes edema of the ankle joint (cyclophosphamide treatment). Apoptosis of tumor cell and inhibition of inflammation can be essential for chemotherapy of malignant and autoimmune diseases.
The aim of the work was to study the effect of modulation of B-lymphocytes activity with cholinotropic drugs on the stomach damage caused by water-immersion stress (WIS). The work was performed on male Wistar rats. Atropine (2 mg/kg), methacin (2 mg/kg) and choline alfoscerat (90 mg/kg) were administered 14 days before the WIS which lasted for 5 hours. The number of antibody-producing cells (APC) in spleen and the content of immunoglobulins IgA, IgG, IgM in blood were determined. The rats injected with saline were served as the control. On day 14 after administration of methacin or choline alfoscerat, but not atropine, an increase in the APC content in the spleen was observed comparing with control. At the same time, there was a significant decrease in the number of gastric ulcers. Thus on the model of water-immersion stress (WIS), it has been shown that B-lymphocytes can serve as a new target for the action of cholinotropic drugs, and modulation of their activity can provide effective prevention against stress.
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