Aims: The objective of this work was to design an amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot (PBRR) in plant material and soil. Methods and Results: Specific primers for the detection of the pathogen were designed based on an amplified region using AFLPs. The banding patterns by AFLPs showed that isolates from diseased roots were clearly distinguishable from others members of the F. solani species complex. Many bands were specific to F. solani PBRR, one of these fragments was selected and sequenced.
Soybean is one of the most economically important crops in Argentina and Brazil. However, there is limited information on the biodiversity of the FGSC from soybean as compared to other crops of large-scale growing such as wheat and maize. A phylogenetic recognition of the Fusarium graminearum species complex (FGSC) isolated from soybean in Argentina and Brazil was performed in order to identify species responsible for trichothecene production. Sequences of genes encoding for the partial translation elongation factor, the 3-O-acetyltransferase and a putative reductase were analysed by the Maximum Parsimony method. Although the present study has focused on a limited number of isolates, this is the first report that provides evidence of the presence of at least four species within the FGSC associated with soybean in Argentina: F. graminearum sensu stricto, F. cortaderiae, F. meridionale and F. boothii. In addition, F. graminearum sensu stricto was detected for the first time among Brazilian isolates from soybean.
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