The mutant cdc7-1 is shown here to block UV induced reversion of six different auxotrophic mutations and forward mutations at several genes concerned with adenine biosynthesis in Saccharomyces cerevisiae. Chemical mutagenesis is also drastically reduced. In its effect on mutagenesis cdc7-1 resembles rad6-1. However, in contrast to rad6-1, cdc7-1 does not affect sporulation or mitotic recombination neither is it sensitive to the antifolate drug trimethoprim. It appears to fall in the same epistatic group as rad6-1. Possible explanations for its action are briefly considered.
The interaction of sodium sulphite and sodium benzoate with nucleosides and DNA has been studied in vitro. Reduction in UV-absorbance was consistently noticed. However, no new products result from such interaction. It is likely that our previous observations of the effects of the 2 food preservatives on DNA synthesis and mitotis in Vicia faba root meristems is not due to direct action of the chemicals at the level of genetic material.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.