Mutagenicity testing of herbicides, fungicides and insecticides. I. Chromosome aberrations in Vicia faba.

Njagi, G. D. E.; Gopalan, H. N. B.

doi:10.1508/cytologia.46.169

Cited by 29 publications

(18 citation statements)

References 2 publications

(3 reference statements)

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“…

In both the laboratory and the field, triclopyr has demonstrated highly effective control of many woody plants and broad leaf weeds.

The present investigation was carried out to study the cytological effect of the herbicide Garlon-4 on the process of mitosis in root tips of Allium cepa.

Materials and methods

Pure strains of bulbs of Allium cepa (variety Giza-6) were used as plant test material.

…”

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confidence: 99%

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Cytological effect of the herbicide Garlon-4 on root mitosis of Allium cepa.

1989

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Many cytological studies have been carried out to detect the harmful effect of different pesticides on different plants (Grover and Tyagi 1980, Njagi and Goplan 1981, Mousa 1982, Reddy and Rao 1982, Soriano 1984, Amer and Farah 1985, Amer and Ali 1986, EL-Khodary et al. 1987.The herbicide 3, 5, 6 trichloro-2-pyridinyloxy-acetic acid, is also known as Garlon-4 . The active chemical ingredient in Garlon herbicide is triclopyr.In both the laboratory and the field, triclopyr has demonstrated highly effective control of many woody plants and broad leaf weeds.The present investigation was carried out to study the cytological effect of the herbicide Garlon-4 on the process of mitosis in root tips of Allium cepa. Materials and methodsPure strains of bulbs of Allium cepa (variety Giza-6) were used as plant test material. Roots while intact, were treated with different concentrations of the herbicide, Garlon-4, ranging from 28ppm to 38400ppm for 3, 6, 12 and 24 hours. The beakers containing Gest solutions were agitated after every 30 minutes since the chemical left a settling suspension.Roots were fixed immediately in acetic alcohol (1:3) for 24 hours.Examination of the roots was done in permantent root tip squash preparation stained by the Feulgen technique.Three replicates were preformed for each treatment and the control, and scoring was made from three roots of each replicate. The mitotic index was calculated for each treatment as a number of dividing cells/100cell. The data obtained from the different treatments were statistically analysed. Results and discussionThe herbicide in the present study caused a change in the frequencies of the different stages. Their frequencies depend on the duration of treatement and concentration of the chemical ap plied. The frequency of prophase increased after treating the root tips of Allium cepa with low concentrations.With higher concentrations and long periods of treatement, the frequency of prophase dorpped and an increase in the metaphase frequency was noticed (Table 1). These changes in the stages of mitosis indicate that Garlon affects the relative duration of each stage

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“…

In both the laboratory and the field, triclopyr has demonstrated highly effective control of many woody plants and broad leaf weeds.

The present investigation was carried out to study the cytological effect of the herbicide Garlon-4 on the process of mitosis in root tips of Allium cepa.

Materials and methods

Pure strains of bulbs of Allium cepa (variety Giza-6) were used as plant test material.

…”

mentioning

confidence: 99%

“…Many cytological studies have been carried out to detect the harmful effect of different pesticides on different plants (Grover and Tyagi 1980, Njagi and Goplan 1981, Mousa 1982, Reddy and Rao 1982, Soriano 1984, Amer and Farah 1985, Amer and Ali 1986, EL-Khodary et al 1987.…”

mentioning

confidence: 99%

Cytological effect of the herbicide Garlon-4 on root mitosis of Allium cepa.

1989

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“…As such, many countries have now banned their use to reduce the amount that comes in food chain. Their possible genetic effect is thus worth studying since most of them ultimately find their way into man and other animals (Najagi and Gopalan 1981). With this end in view two organomercuric compounds along with the in organic mercuric chloride, frequently used as fungicides, have been studied on plant following simple Allium test of Levan (1949).…”

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confidence: 99%

Studies on the cytogenetic effect of some mercuric fungicides.

Nandi

1985

CYTOLOGIA

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“…Additionally, methomyl induced micronuclei formation in mice (16), and elevated levels of sister chromatid exchange (SCE) and chromosomal aberrations (CA) in mice and cultured cells (17)(18)(19)(20). As for plants, Vicia faba root tips also demonstrated elevated levels of CA (21,22). Drosophila S2, HeLa, and HEK293 cells showed significantly higher DNA damage and apoptosis after treatment (3).…”

mentioning

confidence: 99%

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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This study evaluated direct and metabolic genotoxic effects caused by Lannate-90 ® , a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L ® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90 ® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90 ® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90 ® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.

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Mutagenicity testing of herbicides, fungicides and insecticides. I. Chromosome aberrations in Vicia faba.

Cited by 29 publications

References 2 publications

Cytological effect of the herbicide Garlon-4 on root mitosis of Allium cepa.

Cytological effect of the herbicide Garlon-4 on root mitosis of Allium cepa.

Studies on the cytogenetic effect of some mercuric fungicides.

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

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