Lactobacillus probiotics have been investigated in periodontitis. However, the effects of the genus Bifidobacterium on periodontitis are hardly known. This study evaluated the effects of the probiotic (PROB) Bifidobacterium animalis subsp. lactis (B. lactis) HN019 as an adjunct to scaling and root planing (SRP) in rats with experimental periodontitis (EP). At baseline, 32 rats were assigned to 4 groups: C (control), PROB, EP-SRP and EP-SRP-PROB. In groups EP-SRP and EP-SRP-PROB, the mandibular first molars of the animals received a ligature. At day 14, the ligatures were removed and SRP was performed. Animals of groups PROB and EP-SRP-PROB were orally administered with 10 mL/day of 109 colony forming units of B. lactis HN019 for 15 days, starting at day 14. Animals were euthanized at day 29. Histomorphometric, microtomographic and immunohistochemical analyses were performed. Microbiological effects of B. lactis on biofilm were also evaluated. Data were statistically analyzed (ANOVA, Tukey; Kruskal-Wallis, Dunn’s; Two-tailed t-test; p<0.05). Group EP-SRP-PROB presented reduced alveolar bone resorption and attachment loss when compared with Group EP-SRP (p<0.05). Group EP-SRP-PROB showed significantly fewer osteoclasts, increased expression of anti-inflammatory cytokines and reduced expression of proinflammatory cytokines compared with Group EP-SRP (p<0.05). B. lactis promoted a higher ratio between aerobic and anaerobic bacteria in biofilm samples (p<0.05). B. lactis HN019 may have a role in the treatment of EP in rats, as an adjunct to SRP.

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

The study investigated the concurrent validity and reliability of the load-velocity relationship to predict the one-repetition maximum (1RM) of the power clean from the knee (PCK), high pull from the knee (HPK), and mid-thigh clean pull (MTCP). For each exercise, 12 participants performed two 1RM sessions tests and two sessions to measure the barbell’s load-velocity relationship at 30, 45, 60, 75, and 90% of 1RM. The velocity recorded at each load was used to establish the linear regression equation and, consequently, to predict 1RM value. A low validity between the 1RM direct test and predicted 1RM was observed for PCK (typical error [TE]=3.96 to 4.50 kg, coefficient of variation [CV]=4.68 to 5.27%, effect size [ES]=-0.76 to -0.58, Bland-Altman bias [BAB]=9.83 to 11.19 kg), HPK (TE=4.58 to 5.82 kg, CV=6.44 to 8.14%, ES=-0.40 to -0.39, BAB=3.52 to 4.17 kg), and MTCP (TE=6.33 to 8.08 kg, CV=4.78 to 6.16%, ES=-0.29 to -0.19, BAB=3.98 to 6.17 kg). Adequate reliability was observed for the 1RM direct test and for the predicted 1RM. However, based on Bland-Altman limits of agreement, lower measurement errors were obtained for the 1RM direct test in comparison to the predicted 1RM for all the exercises. In conclusion, the load-velocity relationship was not able to predict 1RM values with high accuracy in the PCK, HPK, and MTCP. Moreover, the 1RM direct test was the most reliable for PCK, HPK and MTCP.

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Objetivo: compreender o papel da atenção primária à saúde na assistência à gestante com toxoplasmose e à criança com toxoplasmose congênita. Metodologia: Revisão integrativa de literatura, operacionalizada nas bases de dados PUBMED, LILACS, CVSP, MEDLINE, BDEnf e IBECS, e periódicos CAPES relacionadas ao tema. Resultados: Foi obtida amostra de seis artigos, selecionados dentre os artigos completos, em português ou inglês, publicados nos últimos dez anos, com metodologia observacional, e que tivessem maior pertinência temática ao objetivo proposto. Considerações finais: enfatiza-se o papel e a importância da APS no controle, diagnóstico e tratamento da toxoplasmose, especialmente em relação à educação em saúde com a temática envolvendo também a toxoplasmose congênita, prevenção secundária com o diagnóstico precoce da toxoplasmose na gestação e tratamento quando identificado os casos.

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