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An experiment was conducted with chickens to investigate the relationship between the volume of a single insemination of undiluted semen and fertility and hatchability. The fifteen doses of semen ranged from 0\m=.\0002to 0\m=.\15ml. Semen samples were collected with a minimum amount of cloacal fluid dilution and pooled within strain. Twelve White Leghorn hens were inseminated with each of the fifteen doses of semen from each of three strains of males.Although some fertility resulted from the smallest dose, most of the hens were infertile. Only when doses 0\m=.\004ml and larger were used did nearly all hens lay some fertile eggs.There were highly significant differences among strains and doses in the duration of fertility observed. From forty to seventy million spermatozoa per insemination were required for high fertility. Doses larger than this (0\m=.\006 to 0\m=.\01 ml) increased fertility very slightly but the difference was not significant.The number of 'skipped infertile eggs' per bird (defined as the number of infertile eggs laid between the 2nd day after insemination, and the day the last fertile egg was laid) decreased gradually as the semen dosage was increased from 0\m=.\0006to o\m=.\15ml. There were no significant differences among strains in number of skipped infertile eggs, although the most fertile strain (Strain A) had relatively more skipped infertile eggs per bird than each of the other two less fertile breeds (Strains B and C).The interval (days) between the day of insemination and the day the first fertile egg was laid was longer for hens inseminated with the smaller doses than for those inseminated with the larger doses.The hatchability of fertile eggs was not affected either by the dose of the semen inseminated or by the strain of male used to produce the semen.

The distribution of water in the different body compartments of eight sheep of the Marwari breed was determined after imposing the following treatments: watering ad lib., restriction to 75%, 50%, and 25% respectively of normal daily water requirements (each treatment of 5 days' duration, with a 7-day normal drinking regime between treatments), and complete deprivation of water for 3 days. The total body water, the total blood and plasma volumes, and the extracellular, intracellular, and interstitial fluid volumes of the animals began to decrease when the water intake was reduced below 75% of the normal daily requirement. A reduction to 25% normal was similar in its effect to complete deprivation, at which the plasma volume fell by 43% and the extracellular fluid volume by 33%. The findings clearly point to an unusual ability of these animals to maintain circulation even when faced with considerable haemoconcentration.

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