Spermatozoa Concentration in the Semen of Two Breeds of Fowl Estimated by Three Different Methods

Taneja, G. C.; Gowe, R. S.

doi:10.3382/ps.0400608

Cited by 57 publications

(24 citation statements)

References 14 publications

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“…The third strain, the Ottawa Meat Control strain (Strain C), was a synthetic strain of the meat type. Two of these strains (A and C) have been described in earlier papers in greater detail (Gowe, Johnson, Downs, Gibson, Mountain, Strain &Tinney, 1959, andGowe 1961a). All three strains had been randomly bred for at least two generations.…”

Section: Procedures Stock and Managementmentioning

confidence: 94%

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Effect of Varying Doses of Undiluted Semen on Fertility and Hatchability in the Domestic Fowl

Taneja¹,

Gowe²

1962

Reproduction

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An experiment was conducted with chickens to investigate the relationship between the volume of a single insemination of undiluted semen and fertility and hatchability. The fifteen doses of semen ranged from 0\m=.\0002to 0\m=.\15ml. Semen samples were collected with a minimum amount of cloacal fluid dilution and pooled within strain. Twelve White Leghorn hens were inseminated with each of the fifteen doses of semen from each of three strains of males.Although some fertility resulted from the smallest dose, most of the hens were infertile. Only when doses 0\m=.\004ml and larger were used did nearly all hens lay some fertile eggs.There were highly significant differences among strains and doses in the duration of fertility observed. From forty to seventy million spermatozoa per insemination were required for high fertility. Doses larger than this (0\m=.\006 to 0\m=.\01 ml) increased fertility very slightly but the difference was not significant.The number of 'skipped infertile eggs' per bird (defined as the number of infertile eggs laid between the 2nd day after insemination, and the day the last fertile egg was laid) decreased gradually as the semen dosage was increased from 0\m=.\0006to o\m=.\15ml. There were no significant differences among strains in number of skipped infertile eggs, although the most fertile strain (Strain A) had relatively more skipped infertile eggs per bird than each of the other two less fertile breeds (Strains B and C).The interval (days) between the day of insemination and the day the first fertile egg was laid was longer for hens inseminated with the smaller doses than for those inseminated with the larger doses.The hatchability of fertile eggs was not affected either by the dose of the semen inseminated or by the strain of male used to produce the semen.

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Section: Procedures Stock and Managementmentioning

confidence: 94%

“…Immediately after each semen collection period and after the ten ejaculates were thoroughly mixed, three samples of the mixture were taken to estimate spermatozoa number of the pooled sample by means of the optical density technique described by Taneja & Gowe (1961a).…”

Section: Semen Densitymentioning

confidence: 99%

Effect of Varying Doses of Undiluted Semen on Fertility and Hatchability in the Domestic Fowl

Taneja¹,

Gowe²

1962

Reproduction

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“…Sperm motility was assessed as percentage of progressively motile sperms and a drop of the diluted semen was kept on a clean glass slide and cover slip was applied to examine under high power magnification (40×) and was subjectively assessed. The concentration of sperm was estimated by the method described by Taneja and Gowe (1961) using a colorimeter at 540 nm of wavelength. Tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction test was carried out in duplicate tubes and absorbance was measured with a colorimeter (CL 157, Elico Ltd, India) at 570 nm according to Hazary et al (2001).…”

Section: Semen Collection and Evaluationmentioning

confidence: 99%

Evaluation of semen quality in roosters of different age during hot climatic condition

Shanmugam

Vinoth

Rajaravindra

et al. 2014

Animal Reproduction Science

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“…The optical density was determined at a wavelength of 680 nm (Taneja and Gowe, 1961). The following regression equation was constructed to predict the concentration from optical density readings: /= 0-227 + 11-56 x, r = 0-97 (P < 0-01), RSD = 0-62 where x = optical density, y = number of spermatozoa in 10 6 /mm 3 , r = correlation coefficient and RSD = residual standard deviation.…”

Section: Semen Handling Storage and Inseminationmentioning

confidence: 99%

Effect of semen storage time and number of spermatozoa inseminated on the fertility and hatchability of eggs from dwarf broiler breeder hens

Wambeke

1984

British Poultry Science

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Semen from commercial breeder males was diluted two-fold and stored for 6 and 24 h at 2 to 3 degrees C. For each storage period, groups of caged dwarf broiler breeder hens from the same strain were inseminated with 300, 200 or 100 x 10(6) spermatozoa. Three replicates of 15 birds were inseminated per treatment. Control hens were inseminated with 150 x 10(6) fresh, undiluted spermatozoa. Inseminations were performed for 5 consecutive weeks during a first (32 to 36 weeks of age) and for 6 consecutive weeks during a second experimental period (42 to 47 weeks). During weeks 33 to 36 of the first period, only 24 h storage and 100 x 10(6) spermatozoa produced lower (P less than 0.05) hatchability of all eggs set than the control (84.4 compared to 88.6%). During weeks 43 to 47 of the second period, no significant differences between treatments were observed. Embryonic mortality, measured at different periods during incubation, was not affected by the storage time or the number of spermatozoa inseminated.

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Spermatozoa Concentration in the Semen of Two Breeds of Fowl Estimated by Three Different Methods

Cited by 57 publications

References 14 publications

Effect of Varying Doses of Undiluted Semen on Fertility and Hatchability in the Domestic Fowl

Effect of Varying Doses of Undiluted Semen on Fertility and Hatchability in the Domestic Fowl

Evaluation of semen quality in roosters of different age during hot climatic condition

Effect of semen storage time and number of spermatozoa inseminated on the fertility and hatchability of eggs from dwarf broiler breeder hens

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