From November 1989 to April 1993, blood-fed females and unfed adults and nymphs of lxodes scapularis Say were maintained in housings within 4 different habitats on Long Point, Ontario, Canada, to evaluate the effects of habitat on tick development. More fed females survived the winter within the maple forest (75.6%) than the cottonwood dune (36.1%), whereas 52.8-62.0% survived the winter within the 2 remaining habitats. The proportions of females that laid eggs within the maple forest (90.3%), oak savannah (83.9%), and white pine habitats (78.4%) were similar and greater than in the cottonwood dune (53.8%). In each habitat and all years, females began laying eggs during late April or early May. The time of egg deposition was consistent whether females fed in November and overwintered, or fed during April of the subsequent year. Significantly more eggs hatched within the maple forest (96.4%) and white pine (79.3%) than in the oak savannah (3.8%) or cottonwood dune habitats (0.0%). Hatch occurred in mid- to late July each year. The proportion of unfed I. scapularis adults that survived the winter was not significantly different among the 4 habitats. Unfed adults held in the oak savannah and cottonwood dune habitats died by early June, whereas ticks survived until late June or early July within the maple forest and white pine habitats. Unfed nymphs survived an average of 3.4 mo (range, 0.5-5.5) longer than unfed adults. Fed larvae placed in the field from 22 April to 3 July 1992 molted or died that year. In contrast, 66.7 and 100% of fed larvae placed in the field between 15 and 28 July, and after 28 July, respectively, overwintered before molting. More larvae successfully molted before overwintering (46.9%) than did those that overwintered (17.9%). The proportion of larvae that successfully molted was greatest within the maple forest and least within the cottonwood dune. Fed nymphs placed in the field from 22 April to 4 June molted or died in 1992, whereas 53.6 and 99.2% of fed nymphs placed in the field between 17 June and 28 July, and later than 28 July, respectively, overwintered before molting. Over all habitats, the proportion of nymphs that molted successfully was similar for those that overwintered (43.5%) and those that did not (36.0%). The proportion of nymphs that molted successfully was greatest in the maple forest (60.6%) and least within the cottonwood dune (13.3%). Differences in seasonal extremes of vapor pressure deficits among habitat types were likely responsible for habitat-specific differences in survival of I. scapularis. Based on observations on captive I. scapularis, the life cycle of this tick on Long Point is completed in 3 or 4 yr.

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The impact of microclimate and density of hosts for adult ticks on the density of Ixodes scapularis Say was evaluated within 4 habitats on Long Point, Ontario, from 1989-1992. During the period from May to September, mean weekly vapor pressure deficits were greater within the oak savannah and cottonwood dune habitats than at the maple forest and white pine habitats, which were similar. Vapor pressure deficit was likely the major factor affecting the survivorship of eggs and immature tricks in these habitats. Based on drag sampling, I. scapularis adults demonstrated peak activity in April and October of each year. The mean number of I. scapularis adults collected by dragging during the fall or in the spring did not differ significantly within each habitat. The mean number of adults collected also did not differ among tick cohorts within each habitat; however, significantly more adults were collected within the maple forest than in the white pine habitat. The mean number of I. scapularis adults per white-tailed deer, Odocoileus virginianus (Zimmerman), increased from 1989 to 1991 and then decreased in 1992. Significantly more adult I. scapularis infested deer were observed in 1990 than in 1989. Removal of deer in 1989 and 1990 resulted in a calculated decrease of > 100,000 fed female ticks. Although seasonal variation in microclimate within habitats was closely linked with tick survival and partly explains the differences in abundance of I. scapularis among habitats on Long Point, habitat utilization by deer was also a primary factor governing the local abundance of I. scapularis populations.

35 and 49 days post-inoculation (DPI) or post-infestation (P1). At 7 DPI, B. burgdorferi was transmitted from 18 of 24 syringe-inoculated mice and all three tick-infected mice to 1. scapular-is larvae which fed upon them. However, at 21, 35 and 49 DPI, significantly fewer mice were infective. Borrelia burgdorfrri was isolated from tissues of 14 of 22 syringe-inoculated mice about 56 DPI, and from all three tick-infected mice. However, the level of agreement between xenodiagnosis and bacterial culture was no greater than would be expected by chance alone. We also determined if B. burgdorferi infectivity of mice varied in relation to periods of tick feeding in the field. White-footed mice were trapped during April, July and August 1993 from two habitats on Long Point peninsula (Ontario, Canada), where B. burgdorferi is endemic. Mice from each habitat were infested with laboratory-reared I. scapular-is larvae. Ticks from each mouse were subsequently examined by immunofluorescent assay for B. burgdorfrri infection and mice were cultured for B. burgdorferi. None of 3577 1. scapular-is larvae fed on 62 mice captured within the cottonwood dune habitat were infected with B. burgdorferi, although it was isolated from six of these mice. Within the maple forest habitat, 0/24, 8/21 (38%) and 1/21 (5%) mice transmitted B. burgdorferi to I. scapularis larvae during April, July and August, respectively. Most mice from the maple forest with B. burgdorfrri-positive tissues (14/21) were collected during July, although the level of agreement between xenodiagnosis and tissue culture was poor. Because B. burgdorferi infectivity in mice appears to he of short duration, overwintered 1. scapular-is larvae and nymphs may have to feed upon infected hosts at the same time of year in order for a cycle of B. burgdorferi infection to be maintained on Long Point. Infected I. scapular-is nymphs, rather than persistently infected vertebrate hosts, likely serve as the overwintering "reservoir" for B. burgdotferi on Long Point.

Ixodes scapularis Say populations were evaluated within 4 habitats on Long Point, Ontario, from 1990 to 1992 to ascertain whether differences in density of mouse populations within and among habitats were correlated with that of immature I. scapularis populations. I. scapularis immatures were rarely collected by dragging within the cottonwood dune habitat. Significantly more larvae (P < or = 0.05) were collected by drag sampling within the maple forest habitat than in the oak savannah or white pine habitats for the 1989, 1990, and 1991 cohorts, whereas the size of the 1992 larval cohort did not differ significantly among these habitats. Significantly more nymphs were collected by dragging within the maple forest than in the other 2 habitats for all 4 cohorts. Nymphs from the 1989 and 1990 cohort were more abundant within the oak savannah than the white pine habitat, whereas the 1991 and 1992 cohorts were similar. With few exceptions, I. scapularis immatures were most prevalent on white-footed mice, Peromyscus leucopus (Rafinesque), captured within the maple forest > oak savannah > white pine > cottonwood dune, although differences were not significant in all years and in all habitats. The number of mice captured within the 4 habitats was not correlated with the number of I. scapularis larvae or nymphs infesting them. Likewise, the minimum number of mice alive was not significantly correlated with conversion indices of larvae to nymphs or nymphs to adults. Lack of association between mouse availability and relative size of subsequent cohorts of host-seeking ticks suggests that factors other than the size of the mouse populations were responsible for the observed differences in tick abundance among habitats.