We developed an integrated microfluidic platform for instantaneous flow and localized temperature control. The platform consisted of a flow-focusing region for sample delivery and a cross-junction region embedded with a microheater for cell trapping and localized temperature control by using an active feedback control system. We further used it to measure the membrane transport properties of Jurkat cells, including the osmotically inactive cell volume (V) and cell membrane permeabilities to water (L) and to cryoprotective agent (CPA) solutions (dimethyl sulfoxide (DMSO) in this study) (P) at various temperatures (room temperature, 30 °C, and 37 °C). Such characteristics of cells are of great importance in many applications, especially in optimal cryopreservation. With the results, the corresponding activation energy for water and CPA transport was calculated. The comparison of the results from the current study with reference data indicates that the developed platform is a reliable tool for temperature-dependent cell behavior study, which provides valuable tools for general cell manipulation applications with precise temperature control.
In terms of the leap-forward development for education experiment, both the Chinese and English educational experiment have been very successful, while the progress of the children's mathematics education experiment in China has been relatively slow. It is believed that the main reason for this is the lack of an efficient cognitive tool or system that supports students' study and exploration and correspondingly covers the whole curriculum of children's math education. In recent years, basic mathematics education cognitive tools have undergone rapid development, whereas the lack of a common normalization method for continued in-depth inquiry of the relationships among Objects, Algebra, and Geometry (OAG) has led to the fragmentation of students' mathematical knowledge. Thus, it is difficult for students to develop a systematic understanding of mathematics. Therefore this project attempts to construct a Children's Mathematics Exploring System (CMES), which integrates with cognition theory, method, and contents of basic mathematics education to query the essence of the relationships among OAG. Specifically, the functions of CMES include the following: (1) providing cognitive tools with typical characteristics for the relationships among OAG; (2) offering a general normalized open system on which sustainable in-depth research can be conducted and a conversion tool through which the complete textbook unit of the basic mathematics education can be converted to reveal the relationships among OAG; and (3) presenting a set of cognitive theories, methods, and a system of basic mathematics education to further explore the relationships among OAG. This project will accelerate the development of cognitive technology in basic mathematics education, widen its practical applications, reduce the difficulty of mathematics cognition, and finally improve the quality and efficiency of basic mathematics education.INDEX TERMS Children's math education, exploring system, educational technology.
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