Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Fang, Cifeng; Ji, Fujun; Shu, Zhiquan; Gao, Dayong

doi:10.1039/c6lc01523a

Cited by 33 publications

(29 citation statements)

References 71 publications

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“…In that system, cells were carried and measured while being entirely surrounded by fluid. Later, Fang [23] developed a dynamic microfluidic control system using the stagnation point in a flow field to trap individual cells at a similar intersection area. Based on precise flow control, this system could avoid cell damage and could switch extracellular solutions instantly.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

Yang

Shu

et al. 2019

Micromachines

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The Jurkat cell is an immortalized line of human acute lymphocyte leukemia cells that is widely used in the study of adoptive cell therapy, a novel treatment of several advanced forms of cancer. The ability to transport water and solutes across the cell membrane under different temperatures is an important factor for deciding the specific protocol for cryopreservation of the Jurkat cell. In this study we propose a comprehensive process for determination of membrane transport properties of Jurkat cell. using a novel microfluidic controlled single cell-trapping system. The osmotic behavior of an individual Jurkat cell to water and dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA), under constant temperature, was recorded under a microscope utilizing the modified microfluidic system. The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time. The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane permeability to water and DMSO at room temperature (22 °C). This model and the calculated parameters can help scientists optimize the cryopreservation protocol for any cell type with optimal cryoprotective agents and cooling rate for future experiments.

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Section: Introductionmentioning

confidence: 99%

Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

Yang

Shu

et al. 2019

Micromachines

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“…Another integrated microfluidic platform with localized temperature control was also developed to determine the temperature dependence of membrane permeability of human acute lymphoblastic leukemia cells. 21 A single cell was trapped in the velocity potential well, which was created by manipulating the stagnation point of the extensional flow. The measurements at 22-37°C were achieved with a wire-patterned microheater.…”

Section: Introductionmentioning

confidence: 99%

A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

et al. 2017

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Semi- and selective permeability is a fundamentally important characteristic of the cell membrane. Membrane permeability can be determined by monitoring the volumetric change of cells following exposure to a non-isotonic environment. For this purpose, several microfluidic perfusion chambers have been developed recently. However, these devices only allow the observation of one single cell or a group of cells that may interact with one another in an uncontrolled way. Some of these devices have integrated on-chip temperature control to investigate the temperature-dependence of membrane permeability, but they inevitably require sophisticated fabrication and assembly, and delicate temperature and pressure calibration. Therefore, it is highly desirable to design a simple single-cell trapping device that allows parallel monitoring of multiple separate, individual cells subjected to non-isotonic exposure at various temperatures. In this study, we developed a pumpless, single-layer microarray with high trap occupancy of single cells. The benchmark performance of the device was conducted by targeting spherical particles of 18.8 μm in diameter as a model, yielding trap occupancy of up to 86.8% with a row-to-row shift of 10–30 μm. It was also revealed that in each array the particles larger than a corresponding critical size would be excluded by the traps in a deterministic lateral displacement mode. Demonstrating the utility of this approach, we used the single-cell trapping device to determine the membrane permeability of rat hepatocytes and patient-derived circulating tumor cells (Brx-142) at 4, 22 and 37°C. The membrane of rat hepatocytes was found to be highly permeable to water and small molecules such as DMSO and glycerol, via both lipid- and aquaporin-mediated pathways. Brx-142 cells, however, displayed lower membrane permeability than rat hepatocytes, which was associated with strong coupling of water and DMSO transport but less interaction between water and glycerol. The membrane permeability data reported here provide new insights into the biophysics of membrane transport such as aquaporin expression and coupling transport of water and solutes, as well as providing essential data for the ultimate goal of biobanking rare cells and precious tissues.

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“…In recent years, a number of microfluidic perfusion chambers have been developed specifically for the measurement of cell membrane permeability. 58,[71][72][73] Heo et al 73 proposed a microfluidic device for the quantitative measurement of oocyte volume during various CPA loading protocols. Stepwise, linear, or complex CPA concentration profiles were created to load CPAs into a trapped oocyte, and the oocyte volumetric response to each profile was measured.…”

Section: Role Of Microfluidics In Cryopreservation Of Gametes and Embmentioning

confidence: 99%

IVF-on-a-Chip: Recent Advances in Microfluidics Technology for In Vitro Fertilization

Weng

2019

SLAS Technology

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In vitro fertilization (IVF) has been one of the most exciting modern medical technologies. It has transformed the landscape of human infertility treatment. However, current IVF procedures still provide limited accessibility and affordability to most infertile couples because of the multiple cumbersome processes and heavy dependence on technically skilled personnel. Microfluidics technology offers unique opportunities to automate IVF procedures, reduce stress imposed upon gametes and embryos, and minimize the operator-to-operator variability. This article describes the rapidly evolving state of the application of microfluidics technology in the field of IVF, summarizes the diverse angles of how microfluidics has been complementing or transforming current IVF protocols, and discusses the challenges that motivate continued innovation in this field.

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Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Cited by 33 publications

References 71 publications

Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

IVF-on-a-Chip: Recent Advances in Microfluidics Technology for In Vitro Fertilization

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