Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli. Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli
Penelitian ini bertujuan mengamati interaksi molekuler inhibisi enzim siklooksigenase-2 oleh kurkumin dan beberapa senyawa analognya secara in silico. Penelitian ini menggunakan tujuh senyawa obat yaitu kurkumin, analog 1, 2, 3, 4, etodolak, dan asam arakidonat. Senyawa penambatan yang digunakan adalah 1PXX yang didapat dari situs protein data bank (PDB). Senyawa agonis yang digunakan adalah asam arakidonat, senyawa antagonis etodolak, sedangkan senyawa ujinya yaitu kurkumin, analog 1, 2, 3, dan 4. Semua senyawa di-docking menggunakan aplikasi ArgusLab 4.0.1. proses docking dilakukan dengan metode ArgusDock. Hasil analisis menunjukkan bahwa energi bebas Gibs (ΔG) kurkumin, analog 2, dan 3 lebih kecil dari pada asam arakidonat. Dari hasil penelitian ini dapat disimpulkan bahwa kurkumin, analog 2, dan 3 mampu menghambat ikatan asam arakidonat.
There are several ways that can be done to improve employee performance, among others, by motivating employees and improving work discipline. Increased motivation and discipline can be pursued by the provision of incentive. This study aims to analyze the influence of incentive on Andalas University's employee motivation and discipline and analyze their perceptions on that allowance. This research was conducted by survey using questionnaire toward 78 educational staffs with civil servant status at rectorate of Andalas University. Data were analyzed quantitatively using descriptive analysis and Structural Equation Modeling (SEM). The results showed that the educational staff perceives that the incentive is feasible, allowances are able to increase productivity and improve welfare. However, they still feel that the application of incentive is not fair yet because it is not based on the actual workload. Based on statistical test, it was found that the incentive had a statistically significant and positive influence on work motivation and employee discipline.
Penelitian ini bertujuan memodelkan interaksi molekuler enzim sarkoplasma retikulum/endoplasmik retikulum Ca+2 (SERCA) oleh kurkumin-artemisin dan turunannya secara in silico. Sebagai sampel digunakan 10 senyawa obat yaitu kurkumin, analog 1, analog 2, analog 3, analog 4, artemisin, dihidroartemisin, artemeter, artesunat dan ATP. Reseptor penambatan yang digunakan adalah 2EAS yang didapat dari situs Protein Data Bank (PDB). Semua ditambatkan menggunakan aplikasi ArgusLab 4.0.1. Proses penambatan dilakukan dengan metode ArgusDock. Hasil penambatan menunjukkan semua senyawa uji mampu berikatan dengan reseptor SERCA. Berdasarkan penelitian ini disimpulkan bahwa kurkumin dan analognya mampu menghambat ikatan ATP-reseptor secara kompetitif yang ditandai dengan energi bebas Gibs (Î"G) yang lebih besar. Hal ini menunjukkan ikatan kurkumin dan analognya lebih stabil dari pada ikatan ATP terhadap reseptornya, sedangkan artemisin dan turunannya mampu berikatan dengan SERCA pada site binding domain ion Fe+2.
Deoxyelephantopin is a lactone sesquiterpene compound that shows toxic effects on some cancer cells, otherwise, it is safe on normal cells. The combination of chemotherapy with this compound is intended to determine its effect in increasing the sensitivity of chemotherapy to MCF-7 cancer cells. Cell viability was determined through the MTT method (3-(4,5-dimethyl thiazol-2-il) -2,5-diphenyltetrazolium bromide) to determine the combined effect, while the number of cell deaths was determined through trypan blue staining. Giving deoxyelephantopin-doxorubicin combination to MCF-7 cells showed a synergistic effect with a CI < 0.7. The number of cells that died in the 1.52x and 2.12x combination treatments was higher than the single doxorubicin treatment each at IC50 and ½ IC50 concentrations, this confirms the synergistic effect of the combination. This research proves that deoxyelephantopin can increase the sensitivity and effectiveness of doxorubicin chemotherapy against MCF-7 breast cancer cells.
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