The efficient partitioning of the 2m plasmid of Saccharomyces cerevisiae at cell division requires two plasmid-encoded proteins (Rep1p and Rep2p) and a cis-acting locus, REP3 (STB). By using protein hybrids containing fusions of the Rep proteins to green fluorescent protein (GFP), we show here that fluorescence from GFP-Rep1p or GFP-Rep2p is almost exclusively localized in the nucleus in a cir ؉ strain. Nuclear localization of GFP-Rep1p and GFP-Rep2p, though discernible, is less efficient in a cir 0 host. GFP-Rep2p or GFP-Rep1p is able to promote the stability of a 2m circle-derived plasmid harboring REP1 or REP2, respectively, in a cir
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