The phylogenetic composition of a bacterial community from a hypertrophic freshwater lake in China was investigated by sequencing cloned 16S rRNA genes. Three hundred and thirty-six bacterial clones from four clone libraries in different months (March, May, July and September in 2004) were classified into 142 operational taxonomic units, most of which were affiliated with bacterial divisions commonly found in freshwater ecosystem, e.g. Alpha-, Beta-, Gamma- and Deltaproteobacteria, Bacteriodetes and Actinobacteria. The results showed that the composition of bacterial community in the July library was the most diverse one. Actinobacteria was the most significant lineage in Lake Taihu, with dominant numbers of operational taxonomic units in the May, July and September libraries. Phylogenetic analysis suggested that 53 sequences were grouped into six novel clusters which may represent specific populations indigenous to the environment. Coverage analyses indicated that the clone libraries could provide a fine inventory of bacterial diversity in the lake.

The stable inheritance of the 2m plasmid in a growing population of Saccharomyces cerevisiae is dependent on two plasmid-encoded proteins (Rep1p and Rep2p), together with the cis-acting locus REP3 (STB). In this study we demonstrate that short carboxy-terminal deletions of Rep1p and Rep2p severely diminish their normal capacity to localize to the yeast nucleus. The nuclear targeting, as well as their functional role in plasmid partitioning, can be restored by the addition of a nuclear localization sequence to the amino or the carboxy terminus of the shortened Rep proteins. Analyses of deletion derivatives of the Rep proteins by using the in vivo dihybrid genetic test in yeast, as well as by glutathione S-transferase fusion trapping assays in vitro demonstrate that the amino-terminal portion of Rep1p (ca. 150 amino acids long) is responsible for its interactions with Rep2p. In a monohybrid in vivo assay, we have identified Rep1p, Rep2p, and a host-encoded protein, Shf1p, as being capable of interacting with the STB locus. The Shf1 protein expressed in Escherichia coli can bind with high specificity to the STB sequence in vitro. In a yeast strain deleted for the SHF1 locus, a 2m circle-derived plasmid shows relatively poor stability.The 2m circle, a relatively small circular plasmid (6,318 bp) present in most common strains of Saccharomyces cerevisiae, has optimized a partitioning system and an amplification system that allow it to be propagated stably in a cell population at a copy number of approximately 60 to 100 per cell (reviewed in reference 2). Genetic analyses suggest that two plasmid-coded proteins, Rep1p and Rep2p, in conjunction with a cis-acting locus STB (also called REP3) contribute to the stability function (16,17,19,23). One plausible mechanism for plasmid stability is that the interaction of Rep1p and Rep2p with the STB element serves to overcome the normal bias in plasmid segregation that tends to favor the mother cell over the daughter cell (22). The evidence for this suspected DNA-protein interaction is quite preliminary and rests almost entirely on the observation that urea-solubilized yeast extracts expressing Rep1p and Rep2p or [cir 0 ] extracts supplemented exogenously with Rep1p and Rep2p can bind STB (14).The need for plasmid amplification arises only if and when there is a decrease in copy number below the steady-state value. Normally, each plasmid molecule is replicated once, and only once, per cell cycle (35), and the daughter molecules are partitioned efficiently at cytokinesis (27). When there is a drop in copy number, the amplification system overrides the cell cycle restriction of a single round of plasmid replication during one S phase. Plasmid amplification is absolutely dependent on the 2m circle Flp site-specific recombination system (33). A currently favored model for amplification proposes the recombinational inversion of a bidirectional replication fork and the resultant double-rolling-circle replication mode as the means for obtaining multiple replicas of the plasmid fro...

Click-through rate (CTR) prediction plays a key role in modern online personalization services. In practice, it is necessary to capture user's drifting interests by modeling sequential user behaviors to build an accurate CTR prediction model. However, as the users accumulate more and more behavioral data on the platforms, it becomes non-trivial for the sequential models to make use of the whole behavior history of each user. First, directly feeding the long behavior sequence will make online inference time and system load infeasible. Second, there is much noise in such long histories to fail the sequential model learning. The current industrial solutions mainly truncate the sequences and just feed recent behaviors to the prediction model, which leads to a problem that sequential patterns such as periodicity or long-term dependency are not embedded in the recent several behaviors but in far back history. To tackle these issues, in this paper we consider it from the data perspective instead of just designing more sophisticated yet complicated models and propose User Behavior Retrieval for CTR prediction (UBR4CTR) framework. In UBR4CTR, the most relevant and appropriate user behaviors will be firstly retrieved from the entire user history sequence using a learnable search method. These retrieved behaviors are then fed into a deep model to make the final prediction instead of simply using the most recent ones. It is highly feasible to deploy UBR4CTR into industrial model pipeline with low cost. Experiments on three real-world large-scale datasets demonstrate the superiority and efficacy of our proposed framework and models.

γδ T cells, a subgroup of T cells based on the γδ TCR, when compared with conventional T cells (αβ T cells), make up a very small proportion of T cells. However, its various subgroups are widely distributed in different parts of the human body and are attractive effectors for infectious disease immunity. γδ T cells are activated and expanded by nonpeptidic antigens (P-Ags), major histocompatibility complex (MHC) molecules, and lipids which are associated with different kinds of pathogen infections. Activation and proliferation of γδ T cells play a significant role in diverse infectious diseases induced by viruses, bacteria, and parasites and exert their potential effector function to effectively eliminate infection. It is well known that many types of infectious diseases are detrimental to human life and health and give rise to high incidence of illnesses and death rate all over the world. To date, there is no comprehensive understanding of the correlation between γδ T cells and infectious diseases. In this review, we will focus on the various subgroups of γδ T cells (mainly Vδ1 T cells and Vδ2 T cells) which can induce multiple immune responses or effective functions to fight against common pathogen infections, such as Mycobacterium tuberculosis, Listeria monocytogenes, influenza viruses, HIV, EBV, and HBV. Hopefully, the gamma-delta T cell study will provide a novel effective way to treat infectious diseases.

The efficient partitioning of the 2m plasmid of Saccharomyces cerevisiae at cell division requires two plasmid-encoded proteins (Rep1p and Rep2p) and a cis-acting locus, REP3 (STB). By using protein hybrids containing fusions of the Rep proteins to green fluorescent protein (GFP), we show here that fluorescence from GFP-Rep1p or GFP-Rep2p is almost exclusively localized in the nucleus in a cir ؉ strain. Nuclear localization of GFP-Rep1p and GFP-Rep2p, though discernible, is less efficient in a cir 0 host. GFP-Rep2p or GFP-Rep1p is able to promote the stability of a 2m circle-derived plasmid harboring REP1 or REP2, respectively, in a cir

No abstract

There are many herbal teas that are found in nature that may be effective at treating the symptoms and also shortening the duration of viral infections. When combating viral infections, T lymphocytes are an indispensable part of human acquired immunity. However, studies on the use of natural products in stimulating lymphocyte-mediated interferon-gamma (IFN-γ) production are very limited. In this study, we found that acteoside, a natural phenylpropanoid glycoside from Kuding Tea, enhanced IFN-γ production in mouse lymphocytes in a dose-dependent manner, particularly in the CD4+ and CD8+ subsets of T lymphocytes. To this end, we suggest that the antiviral activity of acteoside was highly correlated to its inducing ability of IFN-γ production. Mechanistically, the activation of T-bet enhanced the promoter of IFN-γ and subsequently resulted in an increased IFN-γ production in T cells. Collectively, we have found a natural product with the capacity to selectively enhance mouse T cell IFN-γ production. Given the role of IFN-γ in the immune system, further studies to clarify the role of acteoside in inducing IFN-γ and prevention of viral infection are needed.

No abstract