Background:Aeromonas hydrophila cause one of the most important diseases in fishes and lead to economic losses, and may be contaminated human beings.Objectives:The current research aimed to investigate the anti-bacterial activity shown by the extracts prepared from different parts of Olea europea, Myrtus communis, Thymus vulgaris, Rosmarinuis officinalis, and Achillea falcata that grow in Syria against A. hydrophila that causes the most dangerous bacterial diseases in fish.Materials and Methods:The study was performed in four stages: First of all, the presence of A. hydrophila was investigated in 450 Samples of Cyprinus Carpio fish using blood agar, Trypticase soya agar, and Analytical Profile Index (API20E). Secondly, the plants extract was obtained using water, absolute alcohol, then ether using Soxhlet extraction apparatus and rotary vacuum evaporator. Thirdly, the antibacterial activity of some antibiotics on these bacteria was evaluated by disk diffusion method. Finally, the antibacterial effect of the extracts was determined by disk diffusion method.Results:The studied antibiotics showed no antibacterial activity against these bacteria, except amikacin which had an acceptable effectiveness. However, the ethanol extracts of the studied plants revealed different antibacterial effects against A. hydrophila which showed antibiotic resistant. T. vulgaris extract had the strongest effect, whereas O. europea extract had the weakest activity. The water and ether petroleum extracts had no antibacterial activities.Conclusions:Ethanol extracts of the studied plants had different antibacterial effects against antibiotic-resistant A. hydrophila. T. vulgaris had the highest activity, R. officinalis had the second, and M. communis and A. falcate were in the third place, while the O. europea had the weakest antibacterial activity.
Background: Continuous researches on drugs that solve the problem of growing number of resistant bacteria are important. Objectives: This research aims to investigate the effectiveness of anti-bacterial properties shown by the extracts prepared from different parts of Punica granatum L. (punicaceae), grown in Syria, against Pasteurella haemolytica, which are resistant to all studied antibiotics. Materials and Methods: A total number of 504 samples of dead sheep lungs were investigated for detection of P. haemolytica, using blood agar, blue methylene, and biochemical tests (oxidase, catalase, indole, urease). Different parts of P. granatum (pericarp, leaves, flowers, seeds) were extracted by water, absolute alcohol, and ether using soxhlet device and rotary vacuum evaporator. Antibiotic susceptibility testing for P. haemolytica by Kirby-Bauer disk diffusion method was conducted, then the extracts susceptibility test against P. haemolytica was conducted. Results: P. haemolytica has infected 20.63% of the total number of samples. The alcoholic extracts prepared from different parts of P. granatum showed the high antibacterial effectiveness, the pericarp extract was the best, whereas the water and ether petroleum extracts had no antibacterial effectiveness against resistant P. haemolytica. Conclusions: Ethanol extracts of P. granatum (pericarp, leaves, flowers, seeds) have antibacterial effects against P. haemolytica which has shown resistance to all studied antibiotics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.