The Anti-Bacterial Effect of Punica granatum Extracts Against Antibiotic Resistant Pasteurella haemolytica

Abstract

Background: Continuous researches on drugs that solve the problem of growing number of resistant bacteria are important. Objectives: This research aims to investigate the effectiveness of anti-bacterial properties shown by the extracts prepared from different parts of Punica granatum L. (punicaceae), grown in Syria, against Pasteurella haemolytica, which are resistant to all studied antibiotics. Materials and Methods: A total number of 504 samples of dead sheep lungs were investigated for detection of P. haemolytica, using blood agar, blue methylene, and biochemical tests (oxidase, catalase, indole, urease). Different parts of P. granatum (pericarp, leaves, flowers, seeds) were extracted by water, absolute alcohol, and ether using soxhlet device and rotary vacuum evaporator. Antibiotic susceptibility testing for P. haemolytica by Kirby-Bauer disk diffusion method was conducted, then the extracts susceptibility test against P. haemolytica was conducted. Results: P. haemolytica has infected 20.63% of the total number of samples. The alcoholic extracts prepared from different parts of P. granatum showed the high antibacterial effectiveness, the pericarp extract was the best, whereas the water and ether petroleum extracts had no antibacterial effectiveness against resistant P. haemolytica. Conclusions: Ethanol extracts of P. granatum (pericarp, leaves, flowers, seeds) have antibacterial effects against P. haemolytica which has shown resistance to all studied antibiotics.

Anti -Bacterial Activity of Punica granatum Flower Extract against Several Gram-Negative and Positive Bacteria

“…Zone inhibition for these bacteria was 10.5 at minimum and 18 at maximum condition. In the other study on other bacteria Pasteurella haemolytica which also showed the antibacterial effects of P. granatum (pericarp, leaves, flowers, seeds) (20). An old study done in Delhi presented that the flower aqueous extract has antiamoebic activity against E. histolytica at 2mg/ml and 4mg/ml concentrations (21).…”

Evaluations of antidepressant activity of Punica granatum peel extract in albino mice

“…Hence the present study was planned. [7][8][9][10][11][12][13][14][15][16] These phytochemical components contribute wonderful, economic and eco-friendly effects of pomegranate peel extract. 17 The antidepressant activity of Punica granatum is mentioned in the Indian system of traditional medicine, but there is a rare scientific evidence to prove its activity by Shamima et al 18 Hence, the present study is designed to evaluate the antidepressant activity of Punica granatum peel extract using animal models in mice was planned.…”