Heat tolerance is a trait of paramount ecological importance and may determine a species' ability to cope with ongoing climate change. Although critical thermal limits have consequently received substantial attention in recent years, their potential variation throughout ontogeny remained largely neglected. We investigate whether such neglect may bias conclusions regarding a species' sensitivity to climate change. Using a tropical butterfly, we found that developmental stages clearly differed in heat tolerance. It was highest in pupae followed by larvae, adults and finally eggs and hatchlings. Strikingly, most of the variation found in thermal tolerance was explained by differences in body mass, which may thus impose a severe constraint on adaptive variation in stress tolerance. Furthermore, temperature acclimation was beneficial by increasing heat knock-down time and therefore immediate survival under heat stress, but it affected reproduction negatively. Extreme temperatures strongly reduced survival and subsequent reproductive success even in our highly plastic model organism, exemplifying the potentially dramatic impact of extreme weather events on biodiversity. We argue that predictions regarding a species' fate under changing environmental conditions should consider variation in thermal tolerance throughout ontogeny, variation in body mass and acclimation responses as important predictors of stress tolerance.

Understanding how organisms adapt to complex environments lies at the very heart of evolutionary biology and ecology, and is of particular concern in the current era of anthropogenic global change. Variation in ecologically important traits associated with environmental gradients is considered to be strong evidence for adaptive responses. Here, we study phenotypic variation along a latitudinal and an altitudinal cline in 968 field-collected males of the widespread European butterfly Pieris napi. In contrast to our expectations, body size decreased with increasing latitude and altitude, suggesting that warmer rather than cooler conditions may be more beneficial for individual development in this species. Higher altitudes but not latitudes seemed to be associated with increased flight performance, suggesting stronger challenges for flight activity in high-altitude environments (e.g. due to strong wind). Moreover, wing melanization increased while yellow reflectance decreased towards colder environments in both clines. Thus, increased melanization under thermally challenging conditions seems to compromise investment into a sexually selected trait, resulting in a trade-off. Our study, although exclusively based on field-collected males, revealed indications of adaptive patterns along geographical clines. It documents the usefulness of field-collected specimens, and the strength of comparing latitudinal and altitudinal clines to identify traits being potentially under thermal selection.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Dispersal, defined as any movements potentially leading to gene flow, is a major process driving a species’ capacity to cope with human‐induced environmental modifications. However, the dispersal process is multi‐causal, which currently hinders predictions regarding a species’ resilience to global change. We used a multifaceted approach to disentangle the relative importance of a suite of dispersal‐related factors in the butterfly Lycaena tityrus, including condition‐ (morphology and behavior) and context‐ (environmental) dependent factors. Experiments were conducted at an experimental platform dedicated to study dispersal, the Metatron, to record emigration propensity in two‐patch experimental metapopulations under different environmental conditions. The butterflies’ individual condition was subsequently assessed in the laboratory. Individual condition did not generally influence emigration propensity. We detected a significant sex bias in emigration propensity, being generally higher in females than in males, but in a context‐dependent manner. The environmental context affected emigration propensity, which was higher when habitat quality was poor. Our results show that emigration is not random in L. tityrus, but is rather an active process triggered by sex and habitat quality. Our main finding was that individual condition, and particularly flight ability measured by a performance test, was not related to emigration propensity in experimental metapopulations. Our results may have important implications for forecasting future species distributions, as deteriorating environmental contexts are likely to increase emigration whatever the individual condition is.

Extreme weather events such as heat waves are predicted to increase in the course of anthropogenic climate change. Widespread species are exposed to a variety of environmental conditions throughout their distribution range, often resulting in local adaptation. Consequently, populations from different regions may vary in their capacity to deal with challenging conditions such as thermal stress. In this study, we investigated clinal variation in body size, fecundity, and oxidative markers along a pan-European latitudinal gradient in the green-veined white butterfly Pieris napi, and additionally gene expression in German individuals. We exposed butterflies from replicated Italian, German, and Swedish populations to cold, control, or hot temperatures for 24 h. Under hot conditions, molecular chaperones were up-regulated, while oxidative damage remained unaffected and levels of the antioxidant glutathione (GSH) were reduced under cold and hot conditions. Thus, the short-term exposure to heat stress did not substantially affect oxidative balance. Moreover, we found decreased body size and fecundity in cooler compared with warmer regions. Interestingly, oxidative damage was lowest in Swedish animals exhibiting (1) high levels of GSH, (2) low early fecundity, and (3) low larval growth rates. These results suggest that Swedish butterflies have a slower life style and invest more strongly into maintenance, while those from warmer regions show the opposite pattern, which may reflect a 'pace-of-life' syndrome.

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