Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...
A Chagas disease serological study was done from October 1991 to March 1992 and 3419 samples were taken from people who donated blood at 12 county areas of Jalisco, México and 8 urban hospitals, by means of indirect hemagglutination reaction. The results indicate that: 73.5% of the donors were from urban area, 26.5% were from rural areas; 1.28% of the donors (N = 44) were considered infected. Thirty nine of them (1.14) were males and 5 females. According to the above mentioned data, we can confirm that the risk of transmission of Trypanosoma cruzi can occur by blood transfusion and this is potentially latent because of the growing urbanization of Chagas disease.
La Universidad de Guadalajara decidió realizar en 1987 un estudio seroepidemiológico para conocer la prevalencia de la infección por Trypanosoma cruzi en los 124 municipios del Estado de Jalisco, México, en el cual se obtuvieron tasas de 21,6 por 100 habitantes. De diciembre de 1993 a junio de 1994 se realizó un seguimiento de algunos de esos individuos, estudiándose sólo 2669 de ellos, de 50 municipios de áreas rurales del estado. Las causas de no encuesta de los faltantes fueron diversas, estando entre las más importantes la emigración (27,6) y la renuencia (14,8). Encontramos a 476 positivos (17,8%) y 2193 negativos (82,2%). Este segundo estudio nos permitió detectar 80 infectados nuevos (2,9%) lo que representa una tasa de incidencia de 30 por 1000 habitantes. Sin embargo, encontramos una seroconversión en los individuos estudiados, ya que de 1007 individuos que estaban positivos en 1987, 943 de ellos continúan así, mientras, que 64 individuos se negativizaron (2,3%), no siendo esto imputable a fallas de laboratorio, la prevalencia de positividad detectada en ambos estudios fue casi idéntica 18,1 y 17,8 % respectivamente, por lo que nosotros pensamos que las condiciones en que viven los individuos de las localidades rurales del Estado de Jalisco favorecen a que las infecciones continúen.
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