Background:The anti-inflammatory effects of daphnetin (7,8-dihidroxicoumarin) have been well-documented, but the potential of daphnetin as an anticancer agent is controversial and remains insufficiently explored. Material and methods: In this work, we evaluated the in vitro anti-proliferative effect of daphnetin in three cell lines by 3-( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, as well as its in vivo antitumor effect in four different types of mouse tumor. Results: With a correlation between in vitro and in vivo results, the tested cell types have different sensitivity to the compound. The following cell lines are arranged according to the in vitro anti-proliferative potency of daphnetin: B16 melanoma cells (inhibitory concentrations 50 [IC 50 ] = 54 ± 2.8 μM) > mitoxantrone (MXT) breast adenocarcinoma cells (IC 50 = 74 ± 6.4 μM) > C26 colon carcinoma cells (IC 50 = 108 ± 7.3 μM). In vivo, the optimal antitumor dose of daphnetin was 40 mg/kg and the magnitudes of inhibition were the following: B16 tumor (48%) > MXT tumor (40%) > S180 fibrosarcoma tumor (30%) > C26 tumor (20%). Conclusion: Our results indicate that daphnetin might have an impact as adjuvant to improve the effectiveness of conventional chemotherapy.
It is proposed to consider recipient's blood group as allocation criteria to assign tissue; and use human skin allografts on patiens affected with burns over 30% of body surface (according the "rule of the 9").
Artificial skin tissue is needed for the treatment of burns, skin cancer, diabetes, and other skin diseases. Tissue engineering combines polymeric biomaterials and cell cultures to develop tissues such as skin that can be used to heal or recover the damaged area. In this paper we report the development of a poly(L‐lactic acid) scaffold coated with polypyrrole doped with iodine by plasma polymerization. The scaffold was characterized by standard techniques for polymers. Cell cultures of keratinocytes and human fibroblasts derived from skin showed that cells adhere to the scaffolds.
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