Three methods (inverted centrifugation; inverted Centrifugation followed by continuowflow washing in 5 per cent dextrase-saline; and dextran sedimentation followed by continnow-flow washing in 5 per cent dextrase-saliie) were evaluated for the preparation of leukocyte-poor red blood cells. Residual leukocyte contents were 0.32, 0.40, and 0.16 billion per 100 g of erythrocytes and erythrocyte losses were 24, 42, and 35 per cent, respectively, for the three methods. The mean removal of leukocytes per unit after inverted centrifugation was 80 per cent. Continuous-flow washing after inverted centrifugation did not remove additional leukocytes. Dextran sedimentation removed a mean 92 per cent of leukocytes. Residual dextran was not detected in the terniinal wash solution. These findings suggest dextran sedimentation may be useful for preparing leukocyte-poor red blood cells for preventing sensitization to leukocytes in high risk recipients such as transplantation candidates.
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