The skin is the site of inoculation of Leishmania spp. in susceptible hosts, and consequently dermatopathies, especially ulcerative dermatitis, are the main clinical signs observed. The aim of this study was to assess parasitism of the skin (intact and ulcerated) among dogs that were naturally infected by Leishmania spp., through immunohistochemical analysis. Skin fragments (intact and ulcerated) were collected from 13 dogs with positive parasitological (bone marrow aspiration and exfoliative skin) and serological examinations (ELISA S7® Biogene) for Leishmania spp. These samples were processed using the immunohistochemical technique, involving the streptavidin-peroxidase complex. Ulcerative lesions were mainly observed on the elbows (53.84%; 7/13), nostrils (15.38%; 2/13), ears (23.07%; 3/13) and wings of the ilium (7.69%; 1/13). A severe parasite load was detected in 46.15% and 76.92% of the intact and ulcerated skin samples tested, respectively. The parasite load on ulcerated skin was statistically higher than on intact skin (p = 0.0221). These results indicate that the intact and ulcerated skin may host a high parasite load of amastigote forms of Leishmania spp., which can favor the transmission of the parasite.Keywords: Skin, leishmaniasis, immunohistochemistry, reservoir, dog. ResumoA pele é o local de inoculação da Leishmania spp. nos hospedeiros susceptíveis e dessa forma, as dermatopatias, principalmente as dermatites ulcerativas são os principais sinais clínicos observados. O objetivo deste estudo foi avaliar o parasitismo na pele (íntegra e ulcerada) em cães naturalmente infectados por Leishmania spp. através da técnica de imunohistoquímica. Fragmentos de pele (íntegra e ulcerada) foram coletados de 13 cães com diagnóstico parasitológico (aspirado de medula óssea e esfoliação cutânea) e sorológico positivos (ELISA S7® Biogene) para Leishmania spp. Amostras foram processadas por imunohistoquímica pelo complexo estreptoavidina-peroxidase. As lesões ulcerativas foram observadas principalmente nas regiões do cotovelo 53,84% (7/13), narina 15,38% (2/13), orelha 23,07% (3/13) e sobre a asa do ílio 7,69% (1/13). Uma intensa carga parasitária foi detectada 46,15% e 76,92% das amostras de pele íntegra e ulcerada, respectivamente. A carga parasitária na pele ulcerada foi estatisticamente superior à pele íntegra (p = 0,0221). Esses resultados indicam que a pele intacta e ulcerada pode albergar uma intensa carga parasitária de formas amastigotas de Leishmania spp., o que pode favorecer a transmissão do parasita.Palavras-chave: Pele, leishmaniose, imunohistoquímica, reservatório, cão.

Teratogenicity and developmental abnormalities in the offspring of female rats that ingested ethanol extract of Neem plants during pregnancy and lactation period were assessed. Twenty-four female Wistar rats were randomly distributed in control group and in three experimental groups and treated during the 4 th , 5 th , and 6 th day of pregnancy. After birth, the lactating females received, by gavage, 65, 135 and 200 mg kg-1 of Neem ethanol extract, during 15 days. Results show, there was no significant difference in body mass index of neonatal rats in the 4 groups evaluated, whereas mean rate of offspring survival was 79.4%. Hair growth, incisor teeth eruption, ear detachment, eyelid opening, and spontaneous ambulation were similar for all groups. Likewise, physical development and development of motor activity, ambulation, and postural reflexes were similar for all groups. The administration of Neem ethanol extract did not cause any reproductive or systemic toxicity in animals. Results show that, Neem ethanol extract safe at doses 65, 135 and 200 mg kg-1 in pregnant or lactating rats.

Osteosarcoma is the most common malignant bone tumor in dogs, most frequently affecting middleto old-aged animals. It is a tumor of mesenchymal origin with great potential for the development of metastases, and is characterized by its aggressive and invasive behavior. In the axial skeleton, primary osteosarcoma is rare but may occur in the ribs, sternum, vertebrae and skull. This paper reports an atypical case of primary chondroblastic osteosarcoma of the rib with esophageal metastasis in a dog. The patient had muffled heart sounds on auscultation, expiratory dyspnea, and severe dehydration. Thoracic radiography showed generalized enlargement of the heart silhouette, suggestive of pericardial effusion. Because of a suspicion of intrathoracic neoplasia and the poor general state of the patient, euthanasia was advised. Necropsy revealed a neoplastic mass within the thoracic cavity, adhered to a rib and the sternum and pericardial sac, in addition to a metastatic mass in the esophagus. Histopathological examination confirmed the diagnosis of this neoplasia. This report describes an unusual case of primary chondroblastic osteosarcoma of the rib in a dog with metastasis in the esophagus. It is considered unusual because of the location of the tumoral mass inside the thoracic cavity and external adhesion to the pericardial sac. Key words: Thoracic cavity, metastasis, malignant tumor, osteoblasts ResumoO Osteossarcoma é o tumor ósseo maligno mais comum nos cães, acometendo com maior frequência animais de média idade a idosos. É um tumor de origem mesenquimal, com grande potencial para o desenvolvimento de metástases, sendo caracterizado pelo seu comportamento agressivo e invasivo. No esqueleto axial, o osteossarcoma primário é considerado raro, pode ocorrer nas costelas, esterno, vértebras e ossos e crânio, além disso, também pode ocorrer no osso pélvico. Este trabalho relata um caso atípico de osteossarcoma condroblástico primário de costela, com metástase esofágica, em um cão. O paciente apresentava abafamento dos sons cardíacos à auscultação, dispneia expiratória e grave desidratação. A radiografia torácica evidenciou o aumento generalizado da silhueta cardíaca, sugestiva de efusão pericárdica. Devido a suspeita de neoplasia intratorácica e ao estado geral ruim do paciente foi realizada eutanásia e a necropsia revelou a massa neoplásica dentro da cavidade torácica, aderida à costela, ao esterno e ao saco pericárdico, além de uma massa metastática localizada no esôfago. O exame histopatológico confirmou o diagnóstico desta neoplasia. Este relato trata-se de um caso atípico de osteossarcoma condroblástico primário de costela em cão, com metástase no esôfago, sendo considerado atípico devido a localização da massa neoplásica dentro da cavidade torácica, aderida externamente ao saco pericárdio.