Weanling W/Fu rats were inoculated intraperitoneally with 1 × 106 to 1 × 107 (C58NT)D Gross virus-induced lymphoma cells. Sera obtained 7 days later showed moderate to marked depressions of overall hemolytic activity, C1, C3 and properdin. These changes were not observed in uninjected W/Fu rats or in animals inoculated with syngeneic normal spleen cells. In the latter group and in the tumor-bearing animals, factor B levels were also depressed. Evidence of complement utilization was also observed in the ascitic fluids of the tumor-bearing W/Fu rats.
Twenty-three carbobenzoxy dipeptide esters, carbobenzoxy peptides, and dipeptides of 1-aminocyclopentanecarboxylic acid were synthesized. Rotation, Ri, and electrophoretic migrations are given. The ability to inhibit Sarcoma-180 in mice varied significantly with structure.Cycloaliphatic amino acids have been investigated for their antitumor properties by Ross,1 Connors,2 and Martel3 and their collaborators, with the finding that 1-aminocyclopentanecarboxylic acid (NSC-1026)4 (I) is effective on in mice, and on Walker (rat) Carcinoma-256. Preliminary clinical trials have been reported.5Connors, et al.,2 have evaluated the effect of substitution on the cyclopentane ring and the amino and carboxyl groups, and conclude that limited substitution markedly diminishes antitumor activity. Both C-terminal and N-terminal glycine peptides retained activity in rats, but the phenylalanyl-l-aminocyclopentanecarboxylic acid peptide was inactive.This Laboratory has been concerned with the synthesis of antibacterial and potential antitumor peptides.
Mg++, Mn++ and Co++ activate the C system as judged by the reduction in cobra venom factor induced hemolysis, and cleavage of properdin factor B and C3. The maximum effect requires the addition of 5 to 10 mM of these cations to dialyzed sera. Guinea pig, human and rat sera respond in similar fashion indicating that these divalent cations can trigger the alternative pathway without the addition of other activating agents.
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