Serum Complement Changes in W/Fu Rats Inoculated with Syngeneic (C58NT) D Lymphoma Cells

Abstract

Weanling W/Fu rats were inoculated intraperitoneally with 1 × 10⁶ to 1 × 10⁷ (C58NT)D Gross virus-induced lymphoma cells. Sera obtained 7 days later showed moderate to marked depressions of overall hemolytic activity, C1, C3 and properdin. These changes were not observed in uninjected W/Fu rats or in animals inoculated with syngeneic normal spleen cells. In the latter group and in the tumor-bearing animals, factor B levels were also depressed. Evidence of complement utilization was also observed in the ascitic fluids of the tumor-bearing W/Fu rats.

Effect of Cobra Venom Factor on the Response of Wistar-Furth Rats to the Gross-Virus-Induced (C58NT)D Lymphoma

“…While it is premature to assess the pre cise role of the humoral immune system as a modulator of tumor immunity, the evidence cited in the present report as well as that in others [1,[31][32][33][34][35][36] do provide a basis for the following comments. The in vivo action of CVF is dual in nature.…”

“…Recent studies in this laboratory indicat ed that the growth of Gross-virus-induced (C58NT)D cells [(C58NT)D cells] in 3-weekold Wistar-Furth (W/Fu) rats was asso ciated with significant depressions of several components of the complement (C) system [1], Sera obtained 7 days following the inoc ulation of 1 X 10* (C58NT)D cells in wean ling W/Fu rats contained lower functional and/or antigenic activities of CH50, C1H50, C3, factor B and properdin than the pre challenge specimens. The utilization of C components in this syngeneic rat tumor sys tem was not considered to provide sufficient evidence to distinguish between an ongoing immune event of but trivial significance to the host or a manifestation of a material > These studies were supported by grants No.…”