Ondrus (1) and McKone (2) used SEP-PAK cartridges to separate and concentrate the FD&C food dyes. McKone used liquid chromatography and an ultraviolet detector to identify the dyes, Ondrus used water/methanol solutions to separate a mixture of two dyes.The objective of this study was the development of a simple procedure for the extraction and separation of five FD &C food dyes, [Yellow 5 (Y-5), Yellow 6 (Y-6), Blue 1 (B-l), Red 3 (R-3), and Red 40 (R-40)], using a low pressure column chromatography apparatus. This requires a minimum of equipment and will be useful in high school chemistry classes.This study utilized a modified 6 mL J. T. Baker 10 SPE column (cartridge), a variable low pressure (2-10 lb) source, and variable concentrations of methanol to extract and separate food dyes from mixtures of pure dyes and from foods. a short length of small-bore rubber tubing and a pinch clamp.
A recent article in this Journal1 described a constant flow buret, which we found to be very reliable. A common reagent used in this system is a strong base such as NaOH. The normality of a standardized NaOH solution is subject to change as a result of reaction with CO2 in the air. The exposure of the titrant to the air in the funnel of the original apparatus limits the use of the apparatus to very short periods.The design shown replaces the open funnel with a polypropylene beaker fitted with a large rubber stopper. The stopper was bored to accept the two tubes as shown in the original apparatus.
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