A simple apparatus for harvesting uncultured magnetotactic microorganisms is described. This apparatus consists of a glass container with two openings. A large opening on the topside is used to introduce the sediment and water. The sediment and water are previously stored in loosely capped bottles previously tested for the presence of magnetotactic bacteria. The apparatus is exposed to a properly aligned magnetic field of a homemade coil and the bacteria are removed through the capillary end of the second opening of the container. Harvested bacteria can then be used to ultrastrucutral studies using electron spectroscopic imaging. Large numbers of magnetotactic bacteria consisting of cocci and rod-shaped cells were efficiently collected from different environments. This apparatus is useful for microbiological studies on uncultured magnetotactic bacteria, especially in molecular approaches for phylogenetic investigations that give information on the natural diversity of microbial communities.
Magnetotactic bacteria from aquatic environments were analyzed with the electron spectroscopic imaging technique. Rod-shaped bacteria and cocci were present in most of the samples observed. Magnetotactic multicellular aggregates were also observed at some of the sampling sites. The use of electron spectroscopic imaging allowed the observation of magnetosomes inside magnetotactic microorganisms with exceptional clarity. The number, size, and morphology of magnetosomes, as well as their ultrastructural spatial disposition inside the bacterial cell, could be directly observed and associated with the disposition of flagella of the respective cells.This allowed us to examine the structural relationships between magnetosomes and flagella, which are important components in the mechanisms of magnetotaxis. In disrupted magnetotactic multicellular aggregates, connections between cells were also visualized. We believe this technique will be useful in studying not only magnetotactic bacteria but also other uncultured microorganisms from natural environments.
O estudo objetiva descrever as motivações para a adoção do trade marketing no setor de lojas de conveniência, bem como suas atribuições e posição na estrutura organizacional. Utilizou-se o estudo de casos múltiplos para investigar as motivações e práticas de quatro redes de lojas de conveniência, além de cinco fabricantes indicados pelas redes pesquisadas. Constatou-se que a existência de uma área formal de trade marketing não é condição suficiente para garantir a colaboração entre os fabricantes e os varejistas de lojas de conveniência. As ações de trade marketing são desenvolvidas e negociadas com o franqueador, todavia sua execução ocorre na instância do franqueado. Trata-se, portanto, de uma relação tripartite (tríade) composta por fabricante, franqueador das lojas de conveniência e seus franqueados.
This essay proposes to refine the concept of consumer engagement in the context of brand communities. A comprehensive review of studies addressing the phenomenon of brand community was made. This paper follows the tradition of Marketing Research and Consumer Behavior, more specifically the perspective of cognitive psychology. The main theoretical foundation of the study is the Social Identity Theory (SIT), also incorporating relevant contributions from the perspective of Consumer Culture Theory (CCT). Therefore, this study contributes to the progress of research on the phenomenon of engagement in brand communities, proposing a theoretical model that relates engagement with its antecedents factors and reflective dimensions.
Resumo Este caso para ensino descreve o desafio de Sergio Lyra, CEO da Team Nogueira, rede de academias de ginástica fundada pelos irmãos Rodrigo e Rogério Nogueira, dois dos maiores lutadores da história do Mixed Martial Arts (MMA) mundial, que haviam transformado seu centro de treinamento em uma academia e, posteriormente, em uma rede de franquias. Em 2015, a Team Nogueira contava com mais de trinta academias por todo o Brasil, no entanto, a desaceleração da popularização da modalidade, aliada à crise econômica que assolava o país e à aposentadoria dos irmãos que davam nome à rede, criavam um cenário de incertezas e preocupações. Esse caso serve de pano de fundo para a discussão sobre a aplicação do marketing no contexto esportivo, permitindo a discussão sobre os desafios relacionados ao alinhamento entre segmentação, seleção do mercado-alvo e posicionamento. Portanto, o caso é recomendado para a disciplina de Fundamentos de Marketing ou Marketing Esportivo em cursos de graduação e pós-graduação, nas sessões dedicadas à discussão sobre segmentação, posicionamento competitivo ou estratégia de marketing.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.