AimTo evaluate the relationship between systemic administration of probiotics and inflammation/resorption processes associated with apical periodontitis (AP) in a rat model.MethodologyTwenty‐four male Wistar rats were used. AP was induced in the mandibular left/right first molars. The animals were arranged into three groups: Control, Lactobacillus rhamnosus and L. acidophilus. Probiotics were orally administered via gavage (109 colony‐forming units (CFU) diluted in 5 mL of water) for 30 days during the development of AP. On the 30th day, blood was collected to analyse the calcium, phosphorus and alkaline phosphatase concentrations in plasma. Then, the animals were euthanized and the jaws removed for micro‐computed tomography and immune‐histopathological analysis for receptor activator of NF‐κB ligand (RANKL), osteoprotegerin (OPG) and tartrate‐resistant acid phosphatase (TRAP). After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn’s test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05).ResultsThere was no significant difference in the calcium and phosphorus levels in plasma amongst the groups (P > 0.05). The level of alkaline phosphatase was significantly higher in the groups that consumed probiotics (P < 0.05). A significantly lower volume of bone resorption was observed in groups that consumed probiotics (P < 0.05). The inflammatory infiltrates and the immunolabelling for RANKL and TRAP were significantly lower in probiotic groups when compared to the control (P < 0.05). Also, the OPG was significantly more immunolabelled in the L. acidophilus group than in the L. rhamnosus and control groups (P < 0.05).ConclusionProbiotic supplementation through gavage (L. rhamnosus and L. acidophilus) had a significant effect on the reduction of inflammation and bone resorption in apical periodontitis development in rats.
Renan DAL-FABBRO (a) Luanna de Oliveira GONÇALVES (a) Alana Sant'Ana do PRADO (a) Flávia Alfredo PLAZZA (a) Naiana Viana VIOLA (b) Luciano Tavares Angelo CINTRA (a) João Eduardo GOMES FILHO (a)
Aim:To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with apical periodontitis in rats.Methodology: Thirty-two three-month-old Wistar rats had apical periodontitis induced in four first molars and were then arranged into four groups: control (C)-rats with apical periodontitis; wine (W)-rats with apical periodontitis receiving 4.28 ml/kg of red wine; resveratrol+quercetin (R+Q)-rats with apical periodontitis receiving 4.28 ml/kg of a solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol and alcohol (ALC)-rats with apical periodontitis receiving the alcoholic dose contained in the wine. The oral gavage treatments were administered daily, from day 0 to day 45. On the 15th day, apical periodontitis was induced, and on the 45th day, the animals were euthanized. Histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β) and micro-computed tomography for bone resorption analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the anova with Tukey's test for parametric data, p < .05. Results:The median score of the inflammatory process was significantly lower in the R+Q group (1) compared to the C (2) (p = .0305) and ALC (3) (p = .0003) groups, and not different from the W (1.5) group. The immunolabeling for OPG was significantly higher in the R+Q group (p = .0054) compared to all groups; the same was observed for IL-10 (p = .0185), different from groups C and ALC. The R+Q group had the lowest TRAP cell count (p < .0001), followed by the W group, both inferior to C and ALC groups. The lowest bone resorption value was in the R+Q group (0.50mm 3 ± 0.21mm 3 ), significantly lower (p = .0292) than the C group (0.88mm 3 ± 0.10mm 3 ). The W group (0.60 mm 3 ± 0.25 mm 3 ) and R+Q group had less bone resorption compared to the ALC group (0.97 mm 3 ± 0.22 mm 3 ), p = .0297 and p = .0042, respectively. Conclusion:Red wine administration to rats for 15 days before induction of apical periodontitis decreased inflammation, TRAP marking and periapical bone resorption compared to alcohol. Resveratrol-quercetin administration reduced the
This case report presents an alternative approach for the removal of a fractured endodontic instrument from the apical third of the root canal. A 52-years-old female patient was referred for specialized endodontic treatment of the maxillary left first molar due to the presence of a periapical lesion and root canal calcification. After clinical and radiographic examination, the calcification was confirmed. During biomechanical preparation, the fracture of a size 25/.06 NiTi reciprocating file (ProDesign R) occurred in the apical third of the palatine canal. The patient was informed about the complication and agreed with the attempt to remove the fragment. Firstly, a slight wear of the dentinal walls around the fragment was made using ultrasonic inserts under magnification. Then, a customized extractor was made using a hypodermic needle and a handling file whose mechanism is similar to the removal method previously described by Masserann. For this, the hypodermic needle was inserted in the root canal and the handling file was adapted in the needle lumen, with the function of fixing the fragment and removing it from the canal through the opposite movement performed by the fractured file. After associating the technique with a hypodermic needle and using ultrasound and an operating microscope to perform the procedure, the fragment was successfully removed. The association of techniques to remove fragments using only items already present in the endodontic arsenal can eliminate the need for surgical treatment and improve the prognosis of endodontic treatment through a safe, simple and cost-effective method that can be performed in the endodontic clinical routine.
This study aimed to evaluate the effectiveness of different Passive Ultrasonic Irrigation (PUI) protocols on debris removal and exposure of dentinal tubules in simulated complex root canal cavities. Twenty single-rooted human mandibular premolars with simulated root canal cavities were filled with the debris and randomly divided into ten groups based on the final irrigation protocol: 1—positive control; 2—negative control; 3—conventional irrigation (CI) with 2.5% sodium hypochlorite (NaOCl); 4—CI with 17% ethylenediaminetetraacetic acid (EDTA) followed by NaOCl; 5—three cycles of PUI for 20 s (NaOCl-NaOCl-NaOCl); 6—three cycles of PUI for 20 s (NaOCl-EDTA-NaOCl); 7—one 60 s PUI cycle (NaOCl); 8—one PUI 180 s cycle (NaOCl); 9—two cycles of PUI for 60 s (EDTA-NaOCl); and 10—two cycles of PUI for 60 s (NaOCl-EDTA). The groups were analyzed by SEM. The Kruskal-Wallis test was used at a 5% level. PUI showed a higher reduction of debris, similar to the positive control group (p > 0.05) and higher than the CI and negative control groups (p < 0.05). Regarding the exposure tubules, the CI groups were similar to the negative control group in all cavities (p > 0.05). The PUI groups were similar to the positive control group (p > 0.05). However, only groups 6, 7, and 10 were statistically different from the CI and negative control (p < 0.05). The protocols using PUI, comprising groups with three cycles of 20 s (NaOCl-EDTA-NaOCl), two cycles of 60 s (EDTA-NaOCl), or one cycle of 60 s (NaOCl), were more effective at removing debris and increasing the exposure of dentinal tubules.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.