Effect of red wine or its polyphenols on induced apical periodontitis in rats

Abstract

Aim:To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with apical periodontitis in rats.Methodology: Thirty-two three-month-old Wistar rats had apical periodontitis induced in four first molars and were then arranged into four groups: control (C)-rats with apical periodontitis; wine (W)-rats with apical periodontitis receiving 4.28 ml/kg of red wine; resveratrol+quercetin (R+Q)-rats with apical periodontitis receiving 4.28 ml/kg of a solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol and alcohol (ALC)-rats with apical periodontitis receiving the alcoholic dose contained in the wine. The oral gavage treatments were administered daily, from day 0 to day 45. On the 15th day, apical periodontitis was induced, and on the 45th day, the animals were euthanized. Histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β) and micro-computed tomography for bone resorption analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the anova with Tukey's test for parametric data, p < .05. Results:The median score of the inflammatory process was significantly lower in the R+Q group (1) compared to the C (2) (p = .0305) and ALC (3) (p = .0003) groups, and not different from the W (1.5) group. The immunolabeling for OPG was significantly higher in the R+Q group (p = .0054) compared to all groups; the same was observed for IL-10 (p = .0185), different from groups C and ALC. The R+Q group had the lowest TRAP cell count (p < .0001), followed by the W group, both inferior to C and ALC groups. The lowest bone resorption value was in the R+Q group (0.50mm 3 ± 0.21mm 3 ), significantly lower (p = .0292) than the C group (0.88mm 3 ± 0.10mm 3 ). The W group (0.60 mm 3 ± 0.25 mm 3 ) and R+Q group had less bone resorption compared to the ALC group (0.97 mm 3 ± 0.22 mm 3 ), p = .0297 and p = .0042, respectively. Conclusion:Red wine administration to rats for 15 days before induction of apical periodontitis decreased inflammation, TRAP marking and periapical bone resorption compared to alcohol. Resveratrol-quercetin administration reduced the

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats

“…Animal models were previously used to assess the mechanisms by which CSI can interfere with the body's response (Carvalho et al, 2006; César‐Neto et al, 2003; Ercan et al, 2019). Also, a rat model for induced AP (Cantiga‐Silva et al, 2021; Cintra et al, 2016; Dal‐Fabbro et al, 2019, 2021) has been used, as rats have dental anatomy and oral microbiota similar to humans (Yoneda et al, 2017).…”

Curcumin reduces inflammation in rat apical periodontitis

“…The analysis was performed considering the perimeter of bone resorption resulting from apical periodontitis, followed by counting OCN‐positive cells or TRAP‐positive multinucleated cells. Finally, the ratio between positive cells and perimeter size was determined (Dal‐Fabbro, Cosme‐Silva, de Rezende Silva Martins Oliveira, et al, 2021; Gomes‐Filho et al, 2015).…”

Role of PI3K in the bone resorption of apical periodontitis

“…The animal experiments were approved by the Ethics Committee of Dalian Medical University (approval number: 2021006). An intraperitoneal injection of Ketamine (90-150 mg/kg) + Xylazine (7.5-16 mg/kg) was administered to anesthetize the mice, then apical periodontitis was established in the mandibular first molar by exposure of the pulp cavity [27]. The mice were fed on standard chow and allowed free access to food and water.…”