The present study was conducted to screen antimicrobial activity of three foliose macrolichens viz., Parmotrema reticulatum (Taylor) M. Choisy (Parmeliaceae), (Nyl.) Trevis (Physciaceae) and Dirinaria consimilis two fruticose macrolichens viz., Ramalina pacifica hossei H. Magn & G. Awasthi (Ramalinaceae) growing on barks of areca trees. The lichens were identified on the basis of morphological, anatomical and metabolites (lichen substances) were identified by thin layer chromatography. Antibacterial activity of lichen extracts was evaluated against three Gram positive and five Gram negative bacteria by Agar well diffusion assay. The lichen extracts were effective in inhibiting all test bacteria. Overall, extract of D. consimilis antibacterial efficacy. Marked inhibitory activity was observed against Antifungal effect of lichen extracts was determined against four molds by Poisoned food technique. Among fungi, the growth of Colletrotrichum capsici by lichen extracts. The observed inhibitory activity of lichens could be ascribed to the presence of lichen substances.
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