Carboxymethyl cellulose (CMC) is a versatile additive whose needs are fulfilled by imports. This becomes an opportunity to develop local CMC products. CMC can be synthesized from the cellulose of oil palm empty fruit bunches (OPEFB). The use of OPEFB as a mixed ingredient of oyster mushroom growing media (baglog) could help the delignification process of OPEFB. Cellulose purified from baglog OPEFB waste using NaOH 10% treatment only produced α-cellulose 80.2% which then being converted to CMC with the purity of 73.4%. Low purity of this CMC did not meet the standard for food-grade which requires purity above 99.5%. This study aimed to improve the purity of cellulose from baglog OPEFB waste by using NaOH 12% treatment. In this way, the purity of the resulting CMC would be expected higher. The resulting CMC product was observed using SEM, FTIR and XRD. The result showed that α-cellulose obtained increased to 84.54% by using 12% NaOH treatment. The resulting CMC had a higher purity level (95.24%). Efforts to increase the degree of substitution and viscosity are still needed to achieve specifications that meet the quality standards of SNI. FTIR and XRD results showed that the characteristics of CMC produced from baglog OPEFB waste were close to commercial CMC as indicated by their functional groups and degree of crystallinity.[Keywords: FTIR, white oyster mushroom, baglog waste, OPEFB, XRD]AbstrakKarboksimetil selulosa(CMC) merupakan zat aditif serbaguna yang kebutuhannyamasihdipenuhi melalui impor. Hal ini menjadi peluang untuk mengembangkan produk CMC lokal.CMC dapat disintesis dari selulosa tandan kosong kelapa sawit (TKKS). Penggunaan TKKS sebagai campuran media pertumbuhan (baglog) jamur tiram putih dapat membantu proses delignifikasi TKKS. Selulosa yang dimurnikan dari limbah TKKS baglog menggunakan perlakuan NaOH 10% hanya menghasilkan α-selulosa sebanyak 80,2%, yang kemudian dikonversi menjadi CMC dengan kemurnian 73,4%. Tingkat kemurnian yang tergolong rendah tersebut tidak memenuhi mutu CMC untuk pangan yang mensyaratkan tingkat kemurnian diatas 99,5%.Penelitian ini bertujuan untuk meningkatkan kemurnian selulosa dari limbah TKKS baglog melalui perlakuan menggunakan NaOH 12%. Dengan cara ini, kemurnian CMC yang dihasilkan diharapkan lebih tinggi. Produk CMC yang dihasilkan diamati menggunakan SEM, FTIR dan XRD. Hasil penelitian menunjukkan bahwa α-selulosa yang diperoleh meningkat menjadi 84,54% pada ekstraksi menggunakan NaOH 12%. CMC yang dihasilkan memiliki tingkat kemurnian yanglebihtinggi, yaitu:95,24%. Upaya untuk meningkatkannilai derajat substitusidan viskositas masih diperlukan untuk mencapai spesifikasi yang memenuhi mutu standar SNI. Hasil FTIR dan XRD menunjukkan bahwa karakteristik CMC yang dihasilkan dari limbah TKKS baglog sudah mendekati CMC komersial ditinjau dari gugus fungsi dan derajat kristalinitasnya. [Kata kunci: FTIR, jamur tiram putih, limbah baglog, TKKS, XRD]
Tandan kosong kelapa sawit (TKKS) berpotensi dimanfaatkan secara integratif dan simultan untuk menghasilkan produk bernilai ekonomis tinggi, antara lain jamur tiram (Pleurotus sp.) dan enzim ligninase (Li-P, Mn-P dan lakase). Sistem teknologi secara simultan menghasilkan jamur konsumsi dan enzim ligninolitik (enzim oksidatif) yang dapat dilakukan dengan sistem fermentasi substrat padat TKKS dengan jamur tiram yang merupakan kelompok jamur pelapuk putih/JPP. Tujuan penelitian ini untuk mengembangkan teknologi integratif pengolahan biomassa pertanian (TKKS) dari perkebunan kelapa sawit secara simultan, mengubah TKKS menjadi barang bernilai jual tinggi melalui produksi jamur tiram dan enzim ligninase. Tahap pertama dilakukan pertumbuhan jamur tiram (putih, kuning, dan pink) pada media TKKS. Pertumbuhan jamur konsumsi dilaksanakan di tempat budidaya jamur tiram milik petani di Cianjur. Kemudian setelah pemanenan jamur tiram, seluruh limbah baglog diekstrak dan dianalisis aktivitas enzim ligninolitiknya. Pertumbuhan jamur tiram pink di media TKKS memiliki Biological Efficiency Ratio (BER) sebesar 52,08%, jamur tiram kuning memiliki BER sebesar 41,67%, dan jamur tiram putih memiliki BER 47,92%. Aktivitas enzim ligninolitik yang di ektraksi dari limbah baglog pertumbuhan jamur tiram meliputi aktivitas enzim Lignin peroksidase (Li-P) sebesar 0,57 U/mL, Mangan peroksidase (Mn-P) sebesar 34,22 U/mL, dan lakase sebesar 0,04 U/mL.
Indonesia produces a large amount of oil palm empty fruit bunch (EFB) that has potency used as media for mushroom cultivation. The research emphasized the use of EFB to grow oyster mushrooms as an alternative for delignification without chemicals. This research aimed to study the growth of oyster mushroom (Pleurotus ostreatus) at EFB based growing media treatments. The media consisted of a mixture of EFB and sawdust at the composition of 0%, 25%, 50%, 75%, and 100%. The media were also supplemented with rice bran, CaCO3, and TSP fertilizer. The data were collected on the mycelium growth and the mushroom weight. During cultivation in baglog, there was no difference in mycelium growth rate. The EFB composition of 25% showed faster growth among the other compositions, followed by the EFB composition of 50%, 75%, and 0%. The highest Biological Efficiency Ratio (BER) at 56.25% was obtained at media composition of 50%. The EFB contained in baglog also showed biodelignification process. Biodelignification has the benefit which can reduce the use of chemicals in the delignification process to convert EFB into cellulose. The highest lignin reduction (40.12%) occurred in 75% of EFB media composition, while hemicellulose (49.56%) occurred in 100% EFB media composition.
Ligninolytic enzymes are known as extracellular enzymes produced by the white rot fungi class of basidiomycetes. One of the most well-known fungi of the white rot fungus isPleurotus ostreatus. The aim of this study to calculate the activity of ligninolytic enzymes in the growth media of Pleurotus ostreatusand their application in decolorization of dye colour. The ligninolytic enzyme extract obtained was used to decolorize bluedyes (MethyleneBlue)and red dyes(Congo Red). The highest laccase enzyme activity was in the first month of 0.35 U/mL with E1 media composition; the highest manganese peroxidase (MnP) enzyme activity was in the fourth month at 31.818 U / mL with E4 media composition; and the highest lignin peroxidase (LiP) enzyme activity was in the fifth month at 0.269 U / mL with E1 media composition. The enzyme extract obtained was then applied to decolorize red and blue dyes. Decolorization of dyes was measured using spectrophotometry with a blue wavelength of 470 nm and red 685 nm. The highest reduction in decolorization of blue dye and red dye was 12 hours with concentration of enzyme addition of 0.5%. Based on these results, ligninolytic enzymes potentiallyto be developed as bioactive agents for detergents.[Keywords: decolorization, laccase, mangan peroxidase, lignin peroxidase, spectrofotometry] AbstrakEnzim ligninolitik dikenal sebagai enzim ekstraseluler yang dihasilkan oleh jamur pelapuk putih golongan basidiomycetes. Salah satu jamur dari golongan jamur pelapuk putih yang banyak dikenal adalah Pleurotus ostreatus. Penelitian ini bertujuan menghitung aktivitas enzim ligninolitik pada media pertumbuhan jamur tiram (Pleurotus ostreatus) dan aplikasinya dalam dekolorisasi zat warna. Ekstrak enzim ligninolitik yang didapatkan kemudian dimanfaatkan untuk dekolorisasi zat warna biru(Methylene Blue)dan merah (Congo Red). Aktivitas enzim lakase tertinggi ada pada bulan pertama sebesar 0,35 U/mL dengan komposisi media E1; aktivitas enzim mangan peroksidase (MnP) tertinggi ada pada bulan keempat sebesar 31,818 U/mL dengan komposisi media E4; dan aktivitas enzim lignin peroksidase (LiP) tertinggi ada pada bulan kelima sebesar 0,269 U/mL dengan komposisi media E1. Ekstrak enzim yang didapat kemudian diaplikasikan untuk dekolorisasi zat warna merah dan biru. Dekolorisasi zat warna diukur menggunakan spektrofotometri dengan panjang gelombang biru pada 470 nm dan merah pada 685 nm. Penurunan dekolorisasi zat warna birudan zat warna merahtertinggi selama 12jam dengan konsentraasi penambahan enzim sebesar 0,5%.Berdasarkan hasil tersebut, enzim ligninolitik sangat potensial untuk dikembangkan sebagai agen bioaktif untuk deterjen.[Kata kunci: dekolorisasi, lakase, mangan peroksidase, lignin peroksidase, spektrofotometri]
Sustainable energy is one of the main challenges of the 21st century. Indonesia is a developing country and ranked fourth in the world population. The total increase in average population growth between 2000 and 2025 is projected at 33.2%. Thus, the problem of energy deficits must be addressed by the Government of Indonesia to overcome the shortage of energy resources in the future. The Indonesian government’s policy on biodiesel began in 2015 and continues to undergo renewal. Starting in September 2018, Indonesia set the B20 rule, and then in January 2020, it began to shift to B30. By the end of 2020, it was targeted to have moved to B50. The concept of sustainability focuses on two things: a combination of environmental and economic considerations. One of the crucial points in sustainable development related to the development of biodiesel B30, B40, and B50 is that economic growth needs to be harmonized with efforts to preserve the environment through long-term maintenance of the availability of biological resources and increasing productivity of the agricultural systems, stability of the human population, limitations on economic growth, and make improvements to the quality of the environment and ecosystem. Biodiesel in Indonesia, Fatty Acid Methyl Ester (FAME) is synthesized from palm oil. Emissions from biodiesel from vegetable oils still contain high NOx gas. When viewed from the side of the impact on the environment, the concept of biodiesel sustainability needs additives/emulsifiers so that the quality and stability of biodiesel increases. That way, the biodiesel effect may show to be environmentally friendly compared to fossil fuels.
White rot fungi Pleurotus ostreatus has long been produced on a large scale for human consumption. This fungi is known to produce ligninolytic enzymes. The aim of this study was to utilize waste fungal medium from empty fruit bunch oil palm (EFBOP) for production of ligninolytic enzymes. Determination of the optimal conditions in this study used the design of statistical experiments Response Surface Method (RSM) with Design Expert® 10 software. Variables used in this research were EFBOP composition (0, 25, 50, 75, 100 %), part baglog (top, middle, bottom), and time of incubation (1, 2, and 3 month). The highest lignin peroxidase activity was 1.72 U/mL obtained on baglog composition with 50% EFBOP the top past of baglog after 2 months incubation. The highest manganese peroxidase activity was 23.00 U/mL obtained on baglog composition with 100% EFBOP at the bottom of baglog after 3 months incubation and the highest laccase activity was 0.14 U/mL on baglog composition with 100% EFBOP the top past of baglog after 1 month.[Keywords: Pleurotus ostreatus, ligninolytic enzyme, fungal medium waste, response surface methodology]. AbstrakJamur pelapuk putih Pleurotus ostreatus telah lama diproduksi skala besar untuk dikonsumsi. Jamur ini diketahui mampu menghasilkan enzim ligninolitik. Selama ini medium limbah produksi P. ostreatus belum dimanfaatkan. Penelitian ini bertujuan untuk memanfaatkan medium limbah produksi jamur tiram yang berbahan dasar tandan kosong kelapa sawit (TKKS) untuk produksi enzim ligninolitik. Penentuan kondisi optimal pada penelitian ini menggunakan desain eksperimen statistika Metode Respons Permukaan (Response Surface Method (RSM)) dengan software Design Expert® 10. Variabel yang digunakan dalam riset ini adalah konsentrasi TKKS (0, 25, 50, 75, 100 %), bagian baglog (atas, tengah, dan bawah), dan waktu inkubasi (1, 2, dan 3 bulan). Aktivitas lignin peroksidase tertinggi diperoleh pada medium dengan komposisi 50% medium pada bagian atas baglog setelah 2 bulan inkubasi dengan aktivitas sebesar 1,72 U/mL. Aktivitas mangan peroksidase tertinggi diperoleh pada medium komposisi 100% TKKS pada bagian bawah baglog setelah 3 bulan inkubasi sebesar 23,00 U/mL, dan lakase tertinggi pada medium komposisi 100% TKKS pada bagian atas baglog setelah 1 bulan inkubasi, yaitu sebesar 0,14 U/mL.[Kata kunci: Pleurotus ostreatus, enzim ligninolitik, limbah media jamur, Metode Respons Permukaan]
Biodiesel in Indonesia is synthesized from palm oil into Fatty Acid Methyl Ester (FAME). In Biodiesel B30, FAME and diesel fuels cannot be homogeneous because FAME is hygroscopic and has a higher density than diesel fuels. This problem can be solved by adding emulsifiers. However, the emulsifier production process is currently limited to the laboratory scale. So, it is necessary to simulate the scaling up emulsifier production system and also the classification of the emulsifier product in the quality control section for this process production. Its integration processes and product are also not following the quality standard. This study aims to identify the relation of emulsifier formulation’s attributes then classify the quality according to the emission of NOx gas. The methods used to determine analyzed system requirements are using BPMN 2.0. The rules are then compiled from the existing emulsifier production system using Association Rules Mining (ARM), and the K-means algorithm will cluster the result. These rules can be used as a reference in taking influential attributes for emulsifier formulation. K-Means algorithm models the rules from the ARM into clusters where data in one cluster has the same characteristics and different characteristics from other clusters. The dataset used is hypothetical data from the formulation and quality testing of the emulsifier. This study’s final results are ten attributes that were approved in the emulsifier formulation and 4 clusters of emulsifier product quality based on NOx gas emissions and separated water layers.
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