Bronchiolitis is the most common cause of lower respiratory tract infection and hospitalization in infancy and some of the patients may require admission to Intensive Care Unit (ICU) due to severe form of the disease. The aim of this study was to determine the risk factors for ICU need. The study group was composed of 30 patients admitted to ICU with the diagnosis of bronchiolitis and 30 bronchiolitis patients that were treated in the regular ward at the same period were enrolled to the study as the control group. Clinical characteristics, laboratory and imaging data were collected and compared statistically. The mean age of the patients was 33.6±24.4 days and 202.8±179.8 days in the study and control groups respectively, p<0.001. The presence of tachypnea at admission (96% of patient group and 70% of control group; p=0.01), having oxygen saturation less than 92% (100% of patient group and 27% of control group; p<0.001), having radiological findings of bronchopneumonia and positive result for respiratory syncytial virus were also found to be significantly different in the study and control groups (p<0.05).
Background: Brucellosis is a highly contagious zoonotic disease that has serious implications on human and animal health. In animals, brucellosis affects reproduction, causing abortion mainly in cows. The aim of this study was to determine the prevalence of brucellosis in different tissues of the bovine fetuses and to analyze whether the amount of the DNA detected changes after formaldehyde treatment and putrefication.Methods & Materials: The material studied was composed of 70 aborted cattle fetuses brought to Adana Veterinary Control and Research Institute during 3-year period. From each fetus, tissues from lung, liver, kidney, heart, spleen and abomasus were analyzed freshly, after 15 days of 10% formaldehyde treatment and after 15 days stay at 20 o C using commercial Brucella genus detection kit on Real-time PCR device (Roche Light Cycler 2.0).Results: Brucella spp. was found positive in all of the tissues of 10 (14%) of the fetuses, lung revealing the highest DNA amount and both putrefication and formaldehyde treatment reduced significantly the DNA that could be detected by PCR, results after formaldehyde being better than putrefied material.
Conclusion:Real-time PCR is safe and sensitive technique for the detection of Brucella in tissues of abortus materials of infected animals.
Various anomalies of chromosome 1 which is the biggest chromosome among all human chromosomes were found in various hematologic diseases. In this retrospective study, clinical features and cytogenetic anomalies of 35 hematological patients with various chromosome 1 anomalies were correlated. Also the effect of chromosome 1 anomalies to the prognosis of those patients was discussed. Conventional cytogenetic analysis of those patients was performed by investigating metaphases of 24 hours stimulated bone marrow samples. After cell culturing, the samples were treated with tripsin and stained with Giemsa (GTG Banding). Analyses were performed on image analysis system (Metasystem, Germany).
Chromosome 1 anomalies were determined in 35 patients (0.5 %) among 6865 samples having done their conventional bone marrow cytogenetic analysis in our center between January 2008 and March 2016. The ratio of chromosome 1 anomalies of totally 701 anomalies among 6865 patients was 4.9 %. Chromosome 1 anomalies were found mostly in patients with MM, MDS and AML in our study group. The most common anomaly was deletion 1 which was seen in 16 (37%) of the patients. Second most common anomaly was derivation 1 which was seen in 13 (30%) of the patients. Also translocations of chromosome 1 with other chromosomes were seen. The genetic aberration formed as a result of chromosomal anomalies result in the formation of hematologic malignancies. The effect on disease pathogenesis and prognosis of some of those anomalies is known and some needs to be investigated and determined.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.