Quantitative analysis of brucella spp in aborted bovine fetuses by real-time PCR

Abstract

Background: Brucellosis is a highly contagious zoonotic disease that has serious implications on human and animal health. In animals, brucellosis affects reproduction, causing abortion mainly in cows. The aim of this study was to determine the prevalence of brucellosis in different tissues of the bovine fetuses and to analyze whether the amount of the DNA detected changes after formaldehyde treatment and putrefication.Methods & Materials: The material studied was composed of 70 aborted cattle fetuses brought to Adana Veterinary Control and Research Institute during 3-year period. From each fetus, tissues from lung, liver, kidney, heart, spleen and abomasus were analyzed freshly, after 15 days of 10% formaldehyde treatment and after 15 days stay at 20 o C using commercial Brucella genus detection kit on Real-time PCR device (Roche Light Cycler 2.0).Results: Brucella spp. was found positive in all of the tissues of 10 (14%) of the fetuses, lung revealing the highest DNA amount and both putrefication and formaldehyde treatment reduced significantly the DNA that could be detected by PCR, results after formaldehyde being better than putrefied material. Conclusion:Real-time PCR is safe and sensitive technique for the detection of Brucella in tissues of abortus materials of infected animals.

Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

“…Previous studies conducted in Turkey revealed that detection rate of Brucella spp. in bovine foetuses varies between 14.3% (10/70) and 41.2% (40/97) (Aslan, Yoldas, Yigin, Demirci, & Saglam, 2016; Buyuk & Sahin, 2011), whereas in sheep foetuses ranges from 18.7% (12/64) to 53.4% (31/58) (Erganis, Kaya, Hadimli, & Guler, 2002; Ica et al., 2012). Furthermore, studies on the prevalence of Campylobacter spp.…”