Human BDCA3 DCs are superior to BDCA1 DCs at antigen cross presentation when delivered to late endosomes and lysosomes but not when delivered to early endosomes.

Significance To navigate large cargo through the viscous cytoplasm, cells use a variety of energy-consuming machines that, akin to ropewalkers, move on intracellular “tracks” in a stepwise “bipedal” fashion. To prevent waste of energy by futile walking, several control mechanisms have evolved. In the case described here for one group of yeast myosins, crystallographic and biophysical analyses revealed that a single myosin molecule associates with an intertwined middle region of two “adaptor” molecules. The adaptor also contains distinct binding sites for cargo. Only when cargo is attached to the myosin-bound adaptor are two of the myosin–adaptor complexes joined into a pair, akin to converting a uniped (unable to walk) into a biped (able to walk).

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