ABSTRAKPenggunaan eksplan mata tunas pisang (Musa paradisiaca var. Raja Bulu) diduga lebih efektif membentuk tunas secara langsung ,sehingga dapat memotong satu tahapan kultur in vitro. Tujuan penelitian ini mengetahui kombinasi hormon dan ukuran eksplan yang terbaik untuk pertumbuhan mata tunas secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial (4x2) dengan 3 kali ulangan. Faktor pertama hormon (P) yaitu P0: tanpa zat pengatur tumbuh, P1: IAA 0,5 mg/l + BA 4,5 mg/l, P2: IAA 3 mg/l + BA 7 mg/l, P3: IAA 5,5 mg/l + BA 9,5 mg/l. Faktor kedua ukuran eksplan yaitu B: mata tunas besar (3 -4 cm) dan K: mata tunas kecil (1 -2 cm). Parameter yang diamati adalah bobot, diameter, warna, morfologi mata tunas dan browning. Data dianalisis dengan Analysis of Variance (ANOVA) dan jika ada beda pengaruh diuji lanjut Duncan'(DMRT) signifikansi 95%. Hasil penelitian menunjukkan terdapat interaksi perlakuan kombinasi hormon dan ukuran mata tunas terhadap diameter mata tunas, namun tidak terdapat interaksi terhadap bobot mata tunas. Perlakuan hormon dan ukuran mata tunas masing -masing berpengaruh nyata terhadap bobot mata tunas. Interaksi hormon IAA 5,5 mg/l + BA 9,5 mg/l dan mata tunas besar menghasilkan diameter mata tunas paling tinggi, sedangkan masing-masing perlakuan menghasilkan bobot mata tunas paling tinggi. Kombinasi hormon IAA 0,5 mg/l + BA 4,5 mg/l menghasilkan diameter paling tinggi pada mata tunas kecil. Semua perlakuan kombinasi hormon dan mata tunas menyebabkan eksplan membengkak dan mengelupas kecuali pada kombinasi hormon IAA 3 mg/l + BA 7 mg/l eksplan hanya membengkak. Warna eksplan menjadi hijau seiring dengan bertambahnya bobot. Kata kunci : mata tunas, Raja Bulu, IAA, BA. ABSTRACTThe use of buds explants pisang (Musa paradisiaca var. Raja Bulu) expected to be more effective that is expected to form shoots directly so as to cut the stage in vitro culture. The purpose of this study to determine the balance of hormones and explant size is best for the growth of the banana king feather buds in vitro. This study uses a Completely Randomized Design (CRD) factorial (4x2) with 3 replications. The first factor hormone (P) ie P0: without growth regulators, P1: IAA 0.5 mg / l + BA 4.5 mg / l, P2: IAA 3 mg / l + BA 7 mg / l, P3: IAA 5.5 mg / l BA + 9.5 mg / l. The second factor is the size of the buds explant ie B: buds large diameter (3-4 cm) and K: buds small diameter (1-2 cm). The parameters were the weight, diameter, color, morphology changes buds and browning. Data were analyzed using Analysis of Variance (ANOVA) and if there are different influences continued of Duncan '(DMRT) 95% significance. The results showed that there is a balance of hormone treatment interaction and the size of the buds to the increase in diameter buds, but there is no interaction against weight gain buds. The treatment of the balance of hormones and the size of the buds each -each significantly affect weight gain buds. Interaction IAA hormone balance of 5.5 mg / l BA + 9.5 mg / l and large buds produce the in...
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