Cystic fibrosis (CF) is the most common genetic disease among Caucasians and is rare among sub-Saharan Africans. The Brazilian population is not ethnically homogeneous but it is the result of threeway ethnic admixture of Europeans, Africans and Amerindians in varying proportions, depending on the region. In the present study, we investigated 33 patients who had been diagnosed and are currently under treatment for CF at the University Hospital João de Barros Barreto, Belém, Pará State. The molecular analysis for G542X, G551D and R553X mutations was performed by PCR followed by RFLP using BstN I, HincII and MboI, respectively, in polyacrylamide gel eletrophoresis and stained with AgNO 3 . The ∆F508 mutation (a deletion of 3 bp) was only analyzed by polyacrylamide gel electrophoresis and stained with AgNO 3 . Each sample was analyzed for regions of interest in the CFTR gene using amplified by PCR and specific primers. The ∆F508 and G551D mutations presented frequencies of 22.7 and 3%, respectively. In 74.3% of the remaining patients, none of the mutations investigated was found. The present study characterized in a sample of patients with an established clinical diagnosis of CF (asthma, repeated bronchopneumonia, disorders of nutritional status, etc.) the most frequent mutation (∆F508) in the North region of Brazil and is also the first report of the G551D mutation. In spite of the wide spectrum of CF mutations and the heterogeneous ethnic origin of the Amazon population, the molecular diagnosis is a helpful additional tool for the diagnosis and treatment of CF patients. Correspondence

The aim of the current study was to assess the activity of Purpureocillium lilacinum and Trichoderma harzianum fungi, either alone or in combination, and the use of a bioactivator to control Pratylenchus brachyurus in soya bean crops. Thus, two experiments were conducted in a greenhouse, and two were conducted in naturally infested fields. Both fungi were efficient in controlling the nematode when they were applied alone, whereas the combination of the two did not improve nematode control. However, the addition of a bioactivator (moss) to the combination of fungi (P. lilacinum + T. harzianum + moss) generally promoted better control of the nematode in the greenhouse, with a reduction in the total abundance of the nematode ranging from 57.1% to 73.75%, and under field conditions, with a reduction ranging from 55.5% to 72.0%. The same treatment increased yield in both field experiments, but the treatment with T. harzianum promoted greater gains in productivity, varying from 11.79% to 20.85%.

No abstract

No presente estudo objetivou-se analisar o teor de macro e micronutrientes de folhas de milho (Zea mays L.) cultivado em solo fertilizado com vermicomposto de lodo de curtume e irrigado com água residuária doméstica. O arranjo dos tratamentos consistiu em um fatorial 2x6, sendo dois tipos de irrigação: água de abastecimento (A) e água residuária doméstica (R); e seis tipos de adubação: T1: Solo controle, sem adubação química e sem vermicomposto; T2: Solo + NPK; T3: Solo + vermicomposto de lodo primário; T4: Solo + P + vermicomposto de lodo primário; T5: Solo + P + vermicomposto de lodo de caleiro e T6: Solo + vermicomposto de lodo de caleiro, em delineamento inteiramente casualizado (DIC), com cinco repetições. Para a análise do tecido foliar coletou-se, de cada planta, a folha inteira oposta e abaixo da primeira espiga (superior), excluída a nervura central por ocasião do aparecimento da inflorescência feminina. Os resultados apontam que tanto a água residuária quanto o vermicomposto de lodo de curtume constituem boa fonte de nutrientes para as plantas de milho, uma vez que os teores de macro e micronutrientes nas folhas mostraram-se satisfatórios, não tendo sido observado nenhum sinal ou sintoma de toxidez. Apesar de certa fragilidade da análise foliar servir como ferramenta exclusiva para avaliação do estado nutricional das plantas, este estudo, pioneiramente, aponta para a potencialidade do uso da água residuária de origem doméstica e de vermicomposto de lodo de curtume (em associação) na cultura do milho, motivando a realização de novas pesquisas.

Keywords: solid waste; recycling; earthworms; sustainable alternatives. INTRODUÇÃOOs processos industriais e atividades humanas, em geral, têm por consequência a geração de resíduos específicos, os quais são constituídos pelas mais diversas substâncias e que, de acordo com a natureza das mesmas, podem ser potencialmente prejudiciais ao ambiente e à saúde humana (KRAEMER, 2006). Conforme discutido por Silva, Souza e Leal (2012), em oposição às comodidades da sociedade moderna, os problemas causados por esses resíduos constituem uma série ameaça