The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo using a gnotobiotic animal model challenged with one of the more sensitive G. vaginalis isolates. A total of 24 isolates from women with and without BV were identified as G. vaginalis. A higher frequency (P,0.05) of this bacterium was observed in women with BV (56.7 %) when compared to healthy women (17.6 %). A total of 86 strains of Lactobacillus were obtained from healthy women and women with BV. Lactobacillus strains were more frequently present (P,0.05) in healthy women (97.5 %) than in women with BV (76.7 %). Lactobacillus crispatus was the predominating strain in both healthy women and women with BV. Lactobacillus jensenii, Lactobacillus johnsonii, Lactobacillus gasseri and Lactobacillus vaginalis were isolated with an intermediate frequency in the two groups. In vitro antagonism assays were performed using as indicators 17 reference strains and the G. vaginalis strains isolated from women with BV and from healthy women. Lactobacillus isolated from healthy women showed the higher antagonistic activity against all the indicator strains when compared with isolates from women with BV. Concerning the indicator strains, G. vaginalis found in women with BV was more resistant to the antagonism, particularly when Lactobacillus isolates from women with BV were used as producer strains. A high vaginal population level of G. vaginalis was obtained by intravaginal inoculation of germ-free mice, and this colonization was accompanied by vaginal histopathological lesions. A tenfold decrease in vaginal population level of G. vaginalis and a reduction of histological lesions were observed when the pathogenic challenge was performed in mice previously monoassociated with an L. johnsonii strain. Concluding, results of the present study suggest that progression of G. vaginalis-associated BV depends in part on a simultaneous presence of Lactobacillus populations with a low antagonistic capacity and of a G. vaginalis strain with a high resistance to this antagonism. The results could also explain why G. vaginalis is frequently found in the vaginal ecosystem of healthy women.
RESUMO:Objetivamos neste artigo problematizar o discurso da "Lei do espanhol". Abordamos também os efeitos de sentido contidos nos dizeres dos sujeitos-professores que nos levaram a depreender representações acerca da lei e seus impactos como política pública, especialmente. O percurso teórico-metodológico foi desenvolvido a partir do corpus de pesquisa constituído por dizeres de professores de espanhol e do texto integral da Lei nº 11.161/2005. Nosso aporte teórico está ancorado na vertente francesa da Análise de Discurso com atravessamento da psicanálise lacaniana. Operamos com a noção de acontecimento e com os conceitos foucaultianos de arquivo e de dispositivo do poder. Nossa investigação permitiu interpretar que essa lei é representada como um mecanismo de poder faltoso. PALAVRAS-CHAVE: língua espanhola; Lei 11.161/2005; representações; análise de discurso; professores E/LE. ABSTRACT:In this article we aim to problematize the discourse of the "Law of Spanish". We also approach the meaning effects contained in the speech of subject-teachers, which led us to deduce representations related especially to the law and its impacts as a public policy. The theoretical and methodological path was developed based on the corpus of the research, which consisted of both Spanish teachers' speech and the integral text of the Law no. 11.161/2005. Our theoretical base is the French Discourse Analysis, interfaced with Lacanian psychoanalysis. We worked with the notions of happening and with the Foucauldian concepts of archive and dispositif of power. Our research has allowed us to interpret that the Law is represented as a faulty mechanism of power.
O artigo versa sobre as representações dos sujeitos-professores de espanhol como língua estrangeira (E/LE) de Belo Horizonte e região metropolitana sobre o ensino de espanhol e seus efeitos de sentido na constituição subjetiva do professor. A pesquisa ancora-se na Análise de Discurso franco-brasileira que parte dos estudos pecheutianos em interface com a psicanálise lacaniana. Em nosso percurso metodológico empregamos como dispositivo de análise dos fatos linguísticos de nosso corpus a interpretação (ORLANDI, 2012), as ressonâncias discursivas (SERRANI-INFANTE, 1998), as noções de intra e interdiscurso (PÊCHEUX, 1974), algumas modalidades da heterogeneidade enunciativa (AUTHIER-REVUZ, 1998, 2004) e noções tomadas da psicanálise. Através dos gestos de interpretação depreendemos significantes que configuram o ensino de E/LE nos circuitos da contingência e da possibilidade.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.