Vinyl chloride (VC), a known human carcinogen, is a common and persistent groundwater pollutant at many chlorinated solvent contaminated sites. The remediation of such sites is challenging because of the lack of knowledge on the microorganisms responsible for in situ VC degradation. To address this, the microorganisms involved in carbon assimilation from VC were investigated in a culture enriched from contaminated site groundwater using stable isotope probing (SIP) and high-throughput sequencing. The mixed culture was added to aerobic media, and these were amended with labeled ((13)C-VC) or unlabeled VC ((12)C-VC). The cultures were sacrificed on days 15, 32, and 45 for DNA extraction. DNA extracts and SIP ultracentrifugation fractions were subject to sequencing as well as quantitative PCR (qPCR) for a functional gene linked to VC-assimilation (etnE). The gene etnE encodes for epoxyalkane coenzyme M transferase, a critical enzyme in the pathway for VC degradation. The relative abundance of phylotypes was compared across ultracentrifugation fractions obtained from the (13)C-VC- and (12)C-VC-amended cultures. Four phylotypes were more abundant in the heavy fractions (those of greater buoyant density) from the (13)C-VC-amended cultures compared to those from the (12)C-VC-amended cultures, including Nocardioides, Brevundimonas, Tissierella, and Rhodoferax. Therefore, both a previously identified VC-assimilating genus (Nocardioides) and novel microorganisms were responsible for carbon uptake. Enrichment of etnE with time was observed in the heavy fractions, and etnE sequences illustrated that VC-assimilators harbor similar Nocardioides-like etnE. This research provides novel data on the microorganisms able to assimilate carbon from VC.

PAES, F. Comparação biomecânica entre CRIF e placa de reconstrução utilizadas para estabilização de fraturas distais de fêmur de cães. [Biomechanical comparison of CRIF and reconstruction plate for stabilization of distal femur fractures in dogs]. 2016. 95 f. Dissertação (Mestrado em Ciências)-Faculdade de

A captive adult male bush dog (Speothos venaticus) was referred to our Veterinary Medical Teaching Hospital from a local zoo due to a two-week history of progressive hind limb gait impairment and ataxia, non-responsive to clinical management. Computed tomography revealed decreased disc space at L3 - L4 level, with probable disc extrusion narrowing the right side of the spinal canal, compressing the spinal cord. We opted to surgically remove the disc material using both fenestration and right lateral pediculectomy (mini-hemilaminectomy) techniques. Twelve days after surgery there was mild residual proprioceptive ataxia. Gait was fully regained with remission of the neurological deficits around the 30th postoperative day. This is - to the best of our knowledge - the first successful report of a lumbar intervertebral disc extrusion in a bush dog (Speothos venaticus) surgically treated by pediculectomy and disc fenestration.

RESUMOObjetivou-se reportar, pela primeira vez descrita até então, a fixação de uma fratura de sétima vértebra lombar (L7) com luxação de L7-S1, por meio de fixação pedicular, utilizando-se Clamp and Rod Internal Fixation (CRIF) em um gato. Um felino de um ano de idade, macho, SRD, foi atendido com histórico de atropelamento havia sete dias, apresentando paraparesia não ambulatória secundária à fratura da sétima vértebra lombar e compressão da cauda equina. Após avaliação geral do paciente, no dia seguinte, ele foi submetido à cirurgia descompressiva por meio de laminectomia dorsal e posterior estabilização pedicular entre L7 e S1, utilizando-se CRIF bilateralmente. O paciente apresentou evolução favorável com deambulação normal após 15 dias do procedimento cirúrgico.Palavras-chave: fratura vertebral, neurocirurgia, gatos ABSTRACTThe aim was to report the establishment of a seventh lumbar vertebra fracture (L7), the first described so far, with L7 -S1 luxation through pedicle fixation using Clamp and Rod Internal Fixation (CRIF) O fator determinante entre optar por um tratamento conservativo ou cirúrgico está em avaliar a instabilidade, sendo mais recentemente avaliada por meio da teoria dos três compartimentos, aplicada inicialmente em pacientes humanos e hoje adaptada para os cães. A teoria divide o corpo vertebral em três http://dx

RESUMOObjetivou-se relatar um caso de protrusão do disco intervertebral com localização atípica e descrever a técnica cirúrgica de corpectomia lateral parcial no tratamento da afecção em um cão. Um cão de sete anos de idade, macho, raça Pug, foi atendido com ataxia proprioceptiva dos membros pélvicos havia três meses, associada à doença do disco intervertebral entre a nona e 10ª vértebras torácicas. Após um mês de tratamento conservativo sem melhora, o paciente foi encaminhado para o tratamento cirúrgico, quando foi realizada corpectomia lateral parcial esquerda para descompressão da medula espinhal. Após a cirurgia, o paciente apresentou melhora considerável do quadro, sendo a técnica eficaz na remoção da compressão extradural, sem manipulação da medula espinhal ou outras complicações.Palavras-chave: compressão medular, corpectomia, neurocirurgia, cães (Wilkens et al., 1996). Em cães não condrodistróficos, principalmente o Pastor Alemão, relata-se herniações entre T2-T3, T3-T4 e T4-T5 (Jäderlund et al., 2002; Gaitero e Añor, 2009), enquanto em raças condrodistróficas há relatos esporádicos de extrusões entre T1-T2 e T9-T10 (Wilkens et al., 1996;Liptak et al., 1999). ABSTRACT The aim of this study is to report a case of intervertebral disc protrusion with atypical location and describe the surgical technique of partial lateral corpectomy in the treatment of this disease in a dogA compressão medular provocada pela DDIV toracolombar em cães necessita de intervenção cirúrgica como principal forma de tratamento e vem sendo tradicionalmente tratada http://dx

Background: Humeral fractures account for 13% of all fractures in cats and commonly affect the middle diaphysis and supracondylar region with comminution. They are usually the result of high energy traumas such as falls, car accidents and firearm injuries. There are few treatment options for these types of fractures and since they are joint injuries, early surgical repair is mandatory to achieve perfect anatomical reduction, to maintain joint congruence and to allow early weight bearing. Our purpose is to report four subacute and chronic fractures of the distal diaphysis associated with comminuted supra and intercondylar fractures, stabilized with transarticular external skeletal fixator in three domestic felines.Cases: (1)- A 3-year-old male docile Siamese cat with 3.7 kg of body weight was presented for consultation with a 14-day history of high rise syndrome after falling from a 12-meter high. It presented distal comminuted humeral fracture that involved the inter and supracondylar regions. It also had right carpal-ulnar subluxation and comminuted right olecranon fracture involving the articular surface in the left thoracic limb. Fracture and joint stabilization was achieved by use of a transarticular hybrid external skeletal fixator on the right front limb. Fracture healing was observed at 150 days postoperatively. (2)- A 10-year-old female aggressive mixed breed cat with 4.1 kg of body weight was presented for consultation with a 16-day history of high rise syndrome after falling from a 9-meter high. The cat had a comminuted right humeral articular fracture with supra and intercondylar involvement and scapular fracture on the contralateral limb. The humerus fracture was treated with a transarticular linear external skeletal fixator and fracture healing was obtained at 240 postoperative days. (3)- A 1-year-old female unsociable mixed breed cat with 4.5 kg of body weight was presented for consultation with a 15-day history of humeral fractures after falling from a 12-meter high building. The cat had comminuted bilateral humeral articular fracture with supra and intercondylar involvement. Fracture stabilization was achieved by use of a transarticular hybrid external skeletal fixator on both limbs. Fracture healing was achieved after 60 days on the right front limb, and after 120 days on the left front limb.Discussion: Patients with distal humeral diaphyseal fractures should be assessed for peripheral nerve injury. None of the reported cases had neurological damage despite having suffered high energy trauma. Surgical approach to humeral distal fractures may be craniolateral, medial or caudal or even combined. In chronic fractures like those in our study, (fractures with more than 14 days of evolution), perfect anatomic reduction wasn’t considered due to presence of fibrous tissue that would require excessive manipulation, leading to injuries to soft tissues and blood supply, what would increase the risk of infection and bone sequestrum. The gold standard for the treatment of articular fractures is rigid internal fixation by means of compressive screws and compressive plates, and they require open reduction. Whenever anatomical reduction can not be performed, due to either the fracture’s evolution period or by the diminished bone length, closed reduction is indicated and stabilization with transarticular fixators is an alternative. Hybrid constructs are well tolerated by patients since they are lighter than circular apparatus and more versatile than linear fixators. In our study, transarticular fixators were used based on the location of the fractures and fracture period. Despite the ankyloses of the humeral-radio-ulnar joint, all patients adapted to the fixator devices very well and presented satisfactory ambulation weight bearing on the first postoperative day already. A disadvantage of the skeletal fixators is the postoperative care, which includes daily dressings, e-collaring, resting and co-operation on behalf of the owner. Two animals presented aggressive/unsocial behavior and still the prolonged postoperative management and adaptation to the fixator were successful. The results suggest that comminuted humeral distal articular fractures can be treated by the use of transarticular external skeletal fixators in cats.

Several types of implants may be used to treat iliac fractures in dogs, of which osteosynthesis with plate is the most described and recommended in the literature. The type of plate and screw are major factors in osteosynthesis success. The use of locking plates has been advocated for this purpose, as they do not allow the plate-bone-screw unit to shift, providing angular stability and rigidity to the system. The present study aimed to describe the unprecedented use of a locking notched head T-plate in the treatment of a caudal body of ilium fracture. A two year old male mongrel dog was attended after falling from a third floor height, with two days of evolution. The animal was in standing position, and during orthopedic examination non-weight bearing lameness of the right hind limb, instability, and crepitus in the right body of ilium, with considerable swelling and pain. The radiographic examination allowed diagnosing a complete fracture of the caudal body of ilium with decrease of the pelvic canal diameter. The patient underwent surgery, and the fracture was stabilized with an LCP notched head T-plate. The patient evolved satisfactorily, with good weight-bearing of the affected limb within 24 hours of surgery, and complete recovery with medical discharge after 60 days of postoperative period, with consolidation of the fracture. Key words: Orthopedics. Traumatology. Pelvis. LCP. ResumoVários tipos de implantes são utilizados no tratamento de fraturas de ílio em cães, sendo a osteossíntese com uso de placa a mais descrita e recomendada pela literatura. O tipo de placa, assim como o tipo de parafuso, são fatores preponderantes no sucesso da osteossíntese. Tem sido defendido o uso de placas bloqueadas, que não permitem movimentação entre a unidade placa-osso-parafuso, conferindo estabilidade angular e rigidez ao sistema. Objetivou-se descrever o uso inédito da placa bloqueada "notched head T-plate" no tratamento de fratura caudal de corpo de ílio. Um cão, macho, sem raça definida, de dois anos de idade foi atendido apresentando histórico de queda de terceiro andar, com dois dias de evolução. O animal apresentava-se em estação e durante a avaliação ortopédica observou-se deambulação com impotência funcional do membro pélvico direito, instabilidade e crepitação em corpo

RESUMO O presente estudo objetivou comparar, biomecanicamente, por meio do ensaio de compressão excêntrica, a resistência dos implantes Clamp and Rod Internal Fixation (CRIF) 5,0mm e placa de reconstrução 3,5mm na fixação de fraturas distais de fêmur de cão. Foram utilizados 22 fêmures de 11 cadáveres de cão entre 2 e 7 anos de idade e peso corporal entre 20 e 40kg e subdivididos em dois grupos, denominados grupo CRIF (GC - 11 fêmures) e grupo placa (GP - 11 fêmures).Para realização dos testes, foi simulada falha que mimetizasse uma fratura distal nos corpos de prova, por meio de uma osteotomia de até 0,5cm, realizada com auxílio de serra oscilatória, imediatamente proximal ao início da tróclea. Os implantes foram fixados segundo os padrões AOSIF, lateralmente ao fêmur, sendo utilizados três parafusos distais e cinco proximais ao foco de fratura. Foi empregado o programa de computador PMI para calcular o ponto máximo de resistência antes da falha e avaliaram-se as variáveis força máxima, deformação máxima real, rigidez, força intermediária e deformação intermediária real. Não foram encontradas diferenças estatisticamente significativas entre os GC e GP quanto às variáveis avaliadas, sugerindo que ambos os implantes são boas opções de fixações para tais fraturas.