Autophagy is an evolutionarily conserved pathway in which cytoplasmic contents are degraded and recycled. This study found that submicromolar concentrations of urolithin A, a major polyphenol metabolite, induced autophagy in SW620 colorectal cancer (CRC) cells. Exposure to urolithin A also dose‐dependently decreased cell proliferation, delayed cell migration, and decreased matrix metalloproteinas‐9 (MMP‐9) activity. In addition, inhibition of autophagy by Atg5‐siRNA, caspases by Z‐VAD‐FMK suppressed urolithin A‐stimulated cell death and anti‐metastatic effects. Micromolar urolithin A concentrations induced both autophagy and apoptosis. Urolithin A suppressed cell cycle progression and inhibited DNA synthesis. These results suggest that dietary consumption of urolithin A could induce autophagy and inhibit human CRC cell metastasis. Urolithins may thus contribute to CRC treatment and offer an alternative or adjunct chemotherapeutic agent to combat this disease.

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Overcoming epidermal growth factor receptor resistance is a critical problem that needs to be solved in clinical practice. Drugs that downregulate the fatty acid synthaseepidermal growth factor receptor will become novel treatments for non-small cell lung cancer. Solanum nigrum, extracted with water at 4 • C, shows strong cytotoxic activity and inhibits tumor growth in Lewis tumor bearing-mice in a dose-dependent manner. A novel active compound in S. nigrum, solaoiacid, was successfully separated and purified from S. nigrum by preparative high-performance liquid chromatography with mass spectrometry and ultra high performance liquid chromatography with timeof-flight tandem mass spectrometry. The IC 50 of solaoiacid on lung cancer cells was 2.3 μmol/L, which was significantly lower than that of the known steroidal glycoalkaloid. Label-free proteomics and STRING Network analysis were used to identify significantly deregulated proteins in lung cancer cells that were treated with the fresh ripe fruit extracts of S. nigrum. S. nigrum regulates multiple signal pathways, including the epidermal growth factor receptor pathway. S. nigrum downregulated 24 main proteins with direct roles in fatty acid biosynthesis. Both S. nigrum and solaoiacid showed strong downregulation of the fatty acid synthase-epidermal growth factor receptor and anti-non-small cell lung cancer effects, and thus will become a novel drug for the treatment of non-small cell lung cancer. K E Y W O R D S epidermal growth factor receptor, fatty acid synthase, non-small cell lung cancer, proteomics, Solanum nigrum berries Article Related Abbreviation: EGFR, epidermal growth factor receptor; FASN, fatty acid synthase; NSCLC, non-small cell lung cancer; Pre-HPLC-MS, preparative liquid chromatography high-performance liquid chromatography-mass spectrometry; SNDA, aqueous extracts of the dried ripe berries of Solanum nigrum; SNFA, aqueous extracts of the fresh ripe Targeted therapy has become the model paradigm of precision medicine in non-small cell lung cancer (NSCLC) berries of Solanum nigrum; SNLH, aqueous extracts of the whole plants of Solanum nigrum L.

The authors describe MnO nanoparticles (NPs) with unique excitation-dependent fluorescence across the entire visible spectrum. These NPs are shown to be efficient optical nanoprobe for multicolor cellular imaging. Synthesis of the NPs is accomplished by a thermal decomposition method. The MnO NPs exhibit a high r relaxivity of 4.68 mM s and therefore give an enhanced contrast effect in magnetic resonance (MR) studies of brain glioma. The cytotoxicity assay, hemolysis analysis, and hematoxylin and eosin (H&E) staining tests verify that the MnO NPs are biocompatible. In the authors' perception, the simultaneous attributes of multicolor fluorescence and excellent MR functionality make this material a promising dual-modal nanoprobe for use in bio-imaging. Graphical abstract A direct method to synthesize fluorescent MnO NPs is reported. The NPs are biocompatible and have been successfully applied for multicolor cellular imaging and MR detection of brain glioma.