The present study aimed to observe the content difference of macrophage migration inhibitory factor [MiF; novoprotein recombinant human MiF (n-6his) (ch33)], TGFβ1 and MMP13 in patients with and without ligamentum flavum (lF) hypertrophy and investigate the roles of MiF in lF hypertrophy. The concentration of MiF, TGFβ1 and MMP13 in lF were detected by eliSa in a lumbar spinal stenosis (lSS) group and a lumbar disc herniation (ldH) group. culture of primary lFs and identification were performed for the subsequent study. cell treatments and cell proliferation assay by ccK-8 was performed. Western blot and quantitative Pcr analysis were used to detect the expression of TGFβ1, MMP13, type i collagen (col-1) and type iii collagen (col-3) and Src which were promoted by MiF. The concentration of MiF, TGFβ1 and MMP13 were higher in the lSS group compared with the LDH group. Culture of primary LFs and identification were performed. Significant difference in LFs proliferation occurred with treatment by MiF at a concentration of 40 nM for 48 h (P<0.05). The gene and protein expression of TGFβ1, MMP13, col-1, col-3 and Src were promoted by MiF (P<0.05). Proliferation of lFs was induced by MiF and MiF-induced proliferation of lFs was inhibited by PP1 (a Src inhibitor). MiF may promote the proliferation of lFs through the Src kinase signaling pathway and can promote extracellular matrix changes by its pro-inflammatory effect. MIF and its mediated inflammatory reaction are driving factors of LF hypertrophy.
Introduction Musculoskeletal injuries (MSIs) are common among U.N. military personnel and cause a substantial toll, but little is known about the actual risks and changes of MSIs. The Chinese level II hospital (CHN L2H) was the highest-level hospital in the Western Sector of the U.N. Mission in South Sudan (UNMISS). This study reviewed and analyzed the MSIs managed by the CHN L2H in UNMISS. Methods Medical records of MSIs in CHN L2H from September 2018 to July 2022 were identified. We analyzed all the MSIs and treatment procedures. Results A total of 857 patients from more than 40 countries were included (86.8% men, average age of 37.83 years), consisting of 457 troop-contributing country, 231 U.N. local, and 169 U.N. international personnel. The most common injury was lumbar muscle strain (14.2%). Sports-related mechanism (29.2%) was the most frequent cause of MSIs. The most significant proportions of anatomical regions were the lumbar spine (22.52%), hand (13.77%), and foot (10.97%). No-steroid anti-inflammatory drugs (43.99%), physical therapy (20.54%), and immobilization (11.32%) were the most used treatments. Conclusions MSIs are common diseases in the CHN L2H in the Western Sector of UNMISS. The universality and complexity of MSIs demonstrate the urgent need to improve prevention, treatment, and rehabilitation.
Objective: To evaluate the efficacy of percutaneous uniplanar pedicle screw (PUPS) combined with injured vertebra fixation for treating thoracolumbar burst fracture and provide technique notes.
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