Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy

Abstract

The present study aimed to observe the content difference of macrophage migration inhibitory factor [MiF; novoprotein recombinant human MiF (n-6his) (ch33)], TGFβ1 and MMP13 in patients with and without ligamentum flavum (lF) hypertrophy and investigate the roles of MiF in lF hypertrophy. The concentration of MiF, TGFβ1 and MMP13 in lF were detected by eliSa in a lumbar spinal stenosis (lSS) group and a lumbar disc herniation (ldH) group. culture of primary lFs and identification were performed for the subsequent study. cell treatments and cell proliferation assay by ccK-8 was performed. Western blot and quantitative Pcr analysis were used to detect the expression of TGFβ1, MMP13, type i collagen (col-1) and type iii collagen (col-3) and Src which were promoted by MiF. The concentration of MiF, TGFβ1 and MMP13 were higher in the lSS group compared with the LDH group. Culture of primary LFs and identification were performed. Significant difference in LFs proliferation occurred with treatment by MiF at a concentration of 40 nM for 48 h (P<0.05). The gene and protein expression of TGFβ1, MMP13, col-1, col-3 and Src were promoted by MiF (P<0.05). Proliferation of lFs was induced by MiF and MiF-induced proliferation of lFs was inhibited by PP1 (a Src inhibitor). MiF may promote the proliferation of lFs through the Src kinase signaling pathway and can promote extracellular matrix changes by its pro-inflammatory effect. MIF and its mediated inflammatory reaction are driving factors of LF hypertrophy.

Modulation of the biological network of lumbar spinal stenosis by Tongdu Huoxue Decoction based on clinical metabolomics

“…Macrophage migration inhibitory factor (MIF) promotes ligamentum flavum proliferation through the Src kinase signaling pathway and extracellular matrix changes through its pro-inflammatory effects. MIF and its mediated inflammatory response are the drivers of ligamentum flavum hypertrophy ( Lu et al, 2022 ). The MIF content is positively correlated with the thickness of the ligamentum flavum, and it promotes fibroblast proliferation and collagen fibrillation which might be an important part of the ligamentum flavum scar repair leading to its hypertrophy.…”

Research on evolution process of full‐layer incision of skin tissue under different laser incidences

“…Type I and Type III proteins have their main distribution in the dermis, while Type IV and Type VII are chiefly located within the subcutaneous tissue [35][36][37][38]. Before delving into a specific analysis of the collagen types and their distribution in samples at various time points as represented in Figure 8, an elementary examination was conducted on the Sirius Red-stained microstructure under a 400Â magnification.…”

“…Additionally, on the seventh day following the second round of laser welding, the thickness of the upper epidermis reached its zenith within the 60 laser group. This suggests that the first round of laser welding at a 90 incident angle effectively triggers an inflammatory response, thus hastening the healing process [35][36][37]. However, the secondary laser welding at a 90 incident angle results in relatively higher thermal damage, which paradoxically hinders epithelial regeneration.…”

Research on evolution process of full-layer incision of skin tissue under different laser incidences

“…Although the healing process was significantly enhanced, it remained challenging to ensure the thickening and hardening of the collagen scaffold post the epithelial regeneration stage. Type I and Type III proteins have their main distribution in the dermis, while Type IV and Type VII are chiefly located within the subcutaneous tissue [47][48][49][50] . Before delving into a specific analysis of the collagen types and their distribution in samples at various time points as represented in Fig.…”

“…Additionally, on the seventh day following the second round of laser welding, the thickness of the upper epidermis reached its zenith within the 60°laser group. This suggests that the first round of laser welding at a 90°incident angle effectively triggers an inflammatory response, thus hastening the healing process [47][48][49] . However, the secondary laser welding at a 90°incident angle results in relatively higher thermal damage, which paradoxically hinders epithelial regeneration.…”