For early cancer diagnosis and treatment, a nanocarrier system is designed and developed with key components uniquely structured at nanoscale according to medical requirements. For imaging, quantum dots with emissions in the near-infrared range (∼800 nm) are conjugated onto the surface of a nanocomposite consisting of a spherical polystyrene matrix (∼150 nm) and the internally embedded, high fraction of superparamagnetic Fe(3)O(4) nanoparticles (∼10 nm). For drug storage, the chemotherapeutic agent paclitaxel (PTX) is loaded onto the surfaces of these composite multifunctional nanocarriers by using a layer of biodegradable poly(lactic-co-glycolic acid) (PLGA). A cell-based cytotoxicity assay is employed to verify successful loading of pharmacologically active drug. Cell viability of human, metastatic PC3mm2 prostate cancer cells is assessed in the presence and absence of various multifunctional nanocarrier populations using the MTT assay. PTX-loaded composite nanocarriers are synthesized by conjugating anti-prostate specific membrane antigen (anti-PSMA) for targeting. Specific detection studies of anti-PSMA-conjugated nanocarrier binding activity in LNCaP prostate cancer cells are carried out. LNCaP cells are targeted successfully in vitro by the conjugation of anti-PSMA on the nanocarrier surfaces. To further explore targeting, the nanocarriers conjugated with anti-PSMA are intravenously injected into tumor-bearing nude mice. Substantial differences in fluorescent signals are observed ex vivo between tumor regions treated with the targeted nanocarrier system and the nontargeted nanocarrier system, indicating considerable targeting effects due to anti-PSMA functionalization of the nanocarriers.

A one-pot colloidal reaction strategy was developed to synthesize asymmetric magnetic composite particles (AMCPs) with high anisotropy and magnetic content. The asymmetricity was established by having two portions of the particle composed of both polystyrene and silica, among which the latter was conjugated with magnetite nanoparticles. Phase separation was found to be critical in developing the asymmetric structure between the inorganic and polymer components during miniemulsion polymerization and the sol-gel reaction. Morphological control of the magnetic composite particles was achieved in a straightforward fashion by adjusting the processing parameters. These asymmetric nanomaterials with superparamagnetic and amphiphilic properties will have significant potential in biomedical applications.

Layer‐by‐layer (LBL) assembly of carbon nanoparticles for low electrical contact resistance thin film applications is demonstrated. The nanoparticles consist of irregularly shaped graphite platelets, with acrylamide/ββ‐methacryl‐oxyethyl‐trimethyl‐ammonium copolymer as the cationic binder. Nanoparticle zeta (ζζ) potential and thereby electrostatic interactions are varied by altering the pH of graphite suspension as well as that of the binder suspension. Film thickness as a function of zeta potential, immersion time, and the number of layers deposited is obtained using Monte Carlo simulation of the energy dispersive spectroscopy measurements. Multilayer film surface morphology is visualized via field‐emission scanning electron microscopy and atomic‐force microscopy. Thin film electrical properties are characterized using electrical contact resistance measurements. Graphite nanoparticles are found to self‐assemble onto gold substrates through two distinct yet overlapping mechanisms. The first mechanism is characterized by logarithmic carbon uptake with respect to the number of deposition cycles and slow clustering of nanoparticles on the gold surface. The second mechanism results from more rapid LBL nanoparticle assembly and is characterized by linear weight uptake with respect to the number of deposition cycles and a constant bilayer thickness of 15 to 21 nm. Thin‐film electrical contact resistance is found to be proportional to the thickness after equilibration of the bilayer structure. Measured values range from 1.6 mΩ cm−2 at 173 nm to 3.5 mΩ cm−2 at 276 nm. Coating volume resistivity is reduced when electrostatic interactions are enhanced during LBL assembly.

Layer-by-layer (LBL) assembly of silica nanoparticles is investigated as a means of controlling the surface wetting properties of gold electroplated onto 316 L stainless-steel substrates while maintaining a low electrical surface contact resistance. The strong polyelectrolyte acrylamide/beta-methacryl-oxyethyl-trimethyl-ammonium copolymer is used as the cationic binder. The impact of silica nanoparticle zeta (zeta) potential for a range of -37.1 to 5.9 mV in the thickness, wettability, and contact resistance of the final LBL-assembled coatings is presented. The zeta potential is varied by altering both the pH and alcohol (ethanol) content of the silica suspensions and polymer suspension, consistent with the predictions of the Debye-Huckel equation. Nanoparticle adsorption is found to occur rapidly, with surface coverage equilibration obtained after only 1 min and uptake that is nearly linear with respect to the number of bilayers deposited. An increase in the absolute value of the (negative) zeta potential in the silica suspension is found to increase the bilayer thickness to an average value as high as 82% of the individual nanoparticle diameter for the smaller nanoparticles investigated, suggesting that nearly complete surface coverage may be achieved after the application of only a single nanoparticle-polymer bilayer (a coating thickness as low as 15.6 nm) and that nanoparticle adsorption is enhanced by electrostatic attraction between substrate and adsorbate. Counterintuitively, a more porous bilayer structure is observed if the zeta potential of the previously deposited nanoparticles is increased while the substrate is immersed in the cationic copolymer suspension, suggesting that copolymer adsorption in inhibited by substrate-solvent interactions. Wetting measurements demonstrate that silica LBL assembly results in a substantial reduction in contact angle from 84 degrees on the bare substrate surface to as low as 15 degrees after the application of a single bilayer and 7 degrees after the application of eight bilayers. A monotonic increase in coating contact resistance is observed with an increase in the thickness with a characteristic volumetric electrical through-plane resistivity of as low as 1.63 kOmega.cm obtained from contact resistance measurement.

No abstract

Quantum dots (QDs) and Fe3O4 nanoparticles were conjugated onto the surfaces of carbon nanotubes (CNTs) for medical diagnosis and treatment. The nanoassembly was designed to meet the specific needs in cancer in vivo imaging and simultaneous treatment. The key functionalities needed for clinical applications were integrated, including CNT surface functionalization for attachment of biological molecules in targeting, drug storage capabilities, fluorescent emissions near the infrared range, and magnetic hyperthermia. CNT-QD-Fe3O4 developed exhibited a strong fluorescence near the infrared region for noninvasive optical in vivo imaging. Magnetization measurements showed nearly reversible hysteresis curves from CNT-QD-Fe3O4 nanoassembly. Fe3O4 conjugated CNT was found to experience hyperthermia heating under alternating electromagnetic field.

No abstract

No abstract