Two-photon fluorescence materials have attracted much recent attention for their many promising applications, especially in the growing field of biomedical imaging. 1-5 Among the best performing two-photon fluorescence materials are semiconductor quantum dots such as CdSe and related core-shell nanoparticles. 6-8 These quantum dots have been demonstrated in various optical imaging experiments in vitro and in vivo. 6,9,10 At the same time, however, heavy metals as the essential elements in available high-performance semiconductor quantum dots have prompted serious health and environmental concerns in the community and beyond. Therefore, the search for benign alternatives has become increasingly important and urgent. Recently, we found and reported 11 that nanosized pure carbon particles may be surfacepassivated to exhibit bright photoluminescence in the visible. These photoluminescent carbon dots (C-Dots, Figure 1a) are of two distinctive features: one is that the underlying carbon particles are very small (sub-10 nm); and the other is that the particle surface is passivated by organic or other molecules via either covalent linkages or chemical adsorption. 11 Mechanistically, the carbon-based photoluminescence has been attributed to passivated defects on the carbon particle surface acting as excitation energy traps. 11,12 Here we report that the C-Dots also exhibit strong luminescence with two-photon excitation in the near-infrared. The estimated two-photon absorption cross-sections of the C-Dots are comparable to those of available high-performance semiconductor quantum dots. In addition, the two-photon luminescence microscopy imaging of the C-Dots internalized in human cancer cells is demonstrated.
There has been significant recent interest in the development of highly fluorescent nanomaterials as contrast agents for optical imaging in vivo. 1 The imaging agents should ideally be bright, nontoxic, biocompatible, and stable against photobleaching. Among the extensively studied are those based on semiconductor quantum dots (QDs) such as CdSe/ZnS. 2 The rationale for the use of QDs over conventional organic dyes is now generally accepted in the literature. 3 There are already successful in vivo imaging demonstrations of QDs on tumor vasculature, tumor-specific membrane antigens, sentinel lymph nodes, etc. 2,4 The semiconductor QDs containing cadmium or other heavy metals are unfortunately known for their significant toxicity even at relatively low concentrations, 5,6 which may prove prohibitive to any patient studies. Therefore, the search for benign alternatives has continued. Of particular interest and significance was the recent finding that small carbon nanoparticles could be surface-passivated by organic or bio-molecules to become strongly fluorescent. 7 These fluorescent carbon nanoparticles, 7,8 dubbed "carbon dots" (C-Dots, Scheme 1), were found to be physicochemically and photochemically stable and non-blinking. The carbon particle core could also be doped with an inorganic salt such as ZnS before the surface functionalization to significantly enhance the fluorescence brightness (C ZnS -Dots, Scheme 1). 9 These carbon dots have been successfully used for in vitro cell imaging with both one-and two-photon excitations. 7,9,10 Carbon is hardly considered as an intrinsically toxic element. Available results from the ongoing toxicity evaluation of the oligomeric PEG-functionalized C-Dots 7 in mice have suggested no meaningful toxic effects, 11 raising the prospect for in vivo biocompatibility and uses of carbon dots. Here we report the first study of carbon dots for optical imaging in vivo. The results suggest that the carbon dots are not only brightly fluorescent in solution, as reported previously, 7,9 but also well-behaved as contrast agents in live mice.The C-Dots and C ZnS -Dots with the PEG diamine, H 2 NCH 2 (CH 2 CH 2 O) n CH 2 CH 2 CH 2 NH 2 (n ∼ 35, PEG 1500N ), as the surface passivation agent were prepared and characterized as previously reported. 7,9,10 Shown in Figure 1 For subcutaneous injection, female DBA/1 mice (∼25 g) were shaved in the back area surrounding the injection point. Upon the injection of a C-Dots solution (30 µg carbon coreequivalent in 30 µL) or a C ZnS -Dots solution (65 µg in 30 µL), the mice were imagined in a Lumazone FA in vivo Imaging System (MAG Biosystems) with 470 nm (FWHM ∼ 40 nm) excitation and 525 nm (FWHM ∼ 47 nm) emission filters. As shown in Figure 2, the fluorescence images of the subcutaneously injected mice exhibited bright emissions from CDots and C ZnS -Dots. The relatively stronger fluorescence from the latter is consistent with the previously reported solution-phase results. 9 The injected carbon dots in mice diffused relatively slowly, with the ...
Fluorescent carbon dots (small carbon nanoparticles with the surface passivated by oligomeric PEG molecules) were evaluated for their cytotoxicity and in vivo toxicity and also for their optical imaging performance in reference to that of the commercially supplied CdSe/ZnS quantum dots. The results suggested that the carbon dots were biocompatible, and their performance as fluorescence imaging agents was competitive. The implication to the use of carbon dots for in vitro and in vivo applications is discussed.
There have been rapid advances in the development and applications of semiconductor quantum dots (QDs) represented by CdSe/ZnS. However, a serious limitation of these QDs is the necessary use of toxic heavy metals. It is reported here that small carbon nanoparticles doped with inorganic salts serve as a highly promising new platform for brightly photoluminescent dots. The photoluminescent carbon dots with the carbon core doped with ZnO (CZnO-Dots) or ZnS (CZnS-Dots) in aqueous solutions are competitive to the commercially available CdSe/ZnS QDs in luminescence brightness.
This progress report provides an overview on recent advances in bioapplications of carbon nanotubes including the chemical modification of carbon nanotubes, targeting specifically their covalent and noncovalent conjugations with a variety of biological and bioactive species (proteins and peptides, DNAs/RNAs, and carbohydrates). Furthermore, the significant recent development and progress in the use of carbon nanotubes for biosensors, drug and other delivery systems, bioimaging, etc. and in the understanding of in vivo biodistribution and toxicity of carbon nanotubes are reported.
Organic-inorganic perovskite solar cells have recently emerged at the forefront of photovoltaics research. Here, for the first time, graphdiyne (GD), a novel two dimension carbon material, is doped into PCBM layer of perovskite solar cell with an inverted structure (ITO/PEDOT:PSS/CH3NH3PbI(3-x)Cl(x)/PCBM:GD/C60/Al) to improve the electron transport. The optimized PCE of 14.8% was achieved. Also, an average power conversion efficiency (PCE) of PCBM:GD-based devices was observed with 28.7% enhancement (13.9% vs 10.8%) compared to that of pure PCBM-based ones. According to scanning electron microscopy, conductive atomic force microscopy, space charge limited current, and photoluminescence quenching measurements, the enhanced current density and fill factor of PCBM:GD-based devices were ascribed to the better coverage on the perovskite layer, improved electrical conductivity, strong electron mobility, and efficient charge extraction. Small hysteresis and stable power output under working condition (14.4%) have also been demonstrated for PCBM:GD based devices. The enhanced device performances indicated the improvement of film conductivity and interfacial coverage based on GD doping which brought the high PCE of the devices and the data repeatability. In this work, GD demonstrates its great potential for applications in photovoltaic field owing to its networks with delocalized π-systems and unique conductivity advantage.
We document the development and simulation characteristics of the next generation modeling system for seasonal to decadal prediction and projection at the Geophysical Fluid Dynamics Laboratory (GFDL). SPEAR (Seamless System for Prediction and EArth System Research) is built from component models recently developed at GFDL—the AM4 atmosphere model, MOM6 ocean code, LM4 land model, and SIS2 sea ice model. The SPEAR models are specifically designed with attributes needed for a prediction model for seasonal to decadal time scales, including the ability to run large ensembles of simulations with available computational resources. For computational speed SPEAR uses a coarse ocean resolution of approximately 1.0° (with tropical refinement). SPEAR can use differing atmospheric horizontal resolutions ranging from 1° to 0.25°. The higher atmospheric resolution facilitates improved simulation of regional climate and extremes. SPEAR is built from the same components as the GFDL CM4 and ESM4 models but with design choices geared toward seasonal to multidecadal physical climate prediction and projection. We document simulation characteristics for the time mean climate, aspects of internal variability, and the response to both idealized and realistic radiative forcing change. We describe in greater detail one focus of the model development process that was motivated by the importance of the Southern Ocean to the global climate system. We present sensitivity tests that document the influence of the Antarctic surface heat budget on Southern Ocean ventilation and deep global ocean circulation. These findings were also useful in the development processes for the GFDL CM4 and ESM4 models.
Quantum of solace: Fluorescent carbon dots (surface‐passivated carbon nanoparticles) are developed as an alternative to classical semiconductor quantum dots. Gel column chromatography afforded carbon dots with emission yields close to 60 %. Their optical properties resemble band‐gap transitions found in nanoscale semiconductors, thus suggesting that nanoscale carbon particles acquire essentially semiconductorlike characteristics.
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