Background: Extra hepatic adverse effects associated with the therapy of chronic HCV infection with sofosbuvir treatment regimens have recently arisen. Objective: This study aimed to assess the inflammatory effect of sofosbuvir and its influence on cellular proliferation, functionality and differentiation of both submandibular (SMGs) and Von Ebner's salivary glands (EGs). Methodology: 21 adult male albino rats were divided into three equal groups: GroupI (control) received orally distilled water; GroupII received orally sofosbuvir (40 mg/kg/day) dissolved in distilled water for one month and GroupIII received sofosbuvir for 2 months. SMGs and EGs sections were processed for H&E, immunohistochemical (using anti-COX-2 and anti-PCNA antibodies) and immunofluorescence (using anti α-amylase antibody) examination. Results: Compared to control group, groupII displayed atrophic changes in SMGs and EGs which were accentuated in groupIII; shrunken acini, glandular cell vacuolization, nuclear degenerative signs, wide degenerative stromal areas and flattening of excretory ductal lining with stagnant secretion as well as the transformation of few serous glandular cells into mucous-like cells particularly in SMGs of groupIII. Likewise, both glands of groupIІ showed significantly increased immunoreactivity to COX-2 in acini and some ductal cells but with a significant decrease in those of groupIII. Regarding PCNA immunoreactivity and α-amylase immunofluorescence, significantly diminished positivity in the glandular cells of both glands in groupII was detected compared to control group whilst insignificant improvement was elucidated in those of groupIII comparing to groupII except for the significant reactivity to α-amylase in EGs of groupIII. Conclusions: It was concluded that the oxidative stress associated degenerative changes caused by sofosbuvir in salivary glands after one month of administration seemed to be diminished after two months of administration due to the body acquired drug tolerance to restore the disturbed physiological processes. Hence, the use of anti-oxidants as an adjuvant treatment could be beneficial.
Background: Chronic aluminum intoxication enhanced the risk for different body tissues in human and animals. Ginger administration may minimize the harmful effects of metal ions toxicity. Objective: This study has attempted to assess and compare the effects of chronic aluminum intoxication and concomitant ginger treatment on different structures of rat periodontium. Methodology: 21 adult male albino rats were divided into three equal groups: For three months; groupI received sterile 0.9% saline/day orally, groups II and III received (20mg/kg/day) aluminum chloride orally in drinking water; then, only groupIII received (150mg/kg/day) ginger oil extract orally for 4 weeks. Some of dissected mandibular halves were used for bone mineral density (BMD) measure. Other halves were decalcified and processed for H&E and immunohistochemical (using anti-CD68+ and anti-osteopontin antibodies) examination. Results: Histopathologically, groupII showed apparently decreased periodontal fibers density, wide degenerative areas and marked inflammatory infiltrates. Likewise, CD68+ was significantly expressed in giant cells in periodontal ligament (PDL) and at resorption surfaces of cementum and bone in groupII. These changes were improved in groupIII with a significant decrease of CD68+ positive cells in PDL. Cementum and bone of groupII presented significant destructive changes that were mostly restored in groupIII. Comparing to groupI, osteopontin expression in groupII significantly increased in acellular cementum(AC)>cellular cementum(CC)>bone> PDL. In comparison, this reactivity was significantly increased in bone>CC>AC in groupIII while osteopontin expression in PDL was mild but with significant increase comparing to groupI. BMD of alveolar bone was highest in groupI>groupIII>groupII with a significant differences.
Conclusion:We concluded that detrimental alterations associated with chronic Al toxicity were markedly detected in PDL>bone>CC>AC that could result in serious clinical outcomes. Ginger extract greatly ameliorated these changes in bone>CC>AC>PDL confirming its strong curative potency against aluminum associated oxidative damage thus it is indicated for use in further new therapeutic approaches.
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