Does stem cells approach ameliorate the prospective alterations of mandibular joint histology and acetylcholinesterase expression in experimentally induced Alzheimer’s disease?

Saad, Fatma Adel; Abdellah, Ahmed

doi:10.21608/ejh.2020.32545.1314

Cited by 1 publication

(1 citation statement)

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“…TMJ-OA has also been the target of MSC therapy, 2 although the majority of studies have focused on the regeneration of TMJ tissues, especially cartilage and sub-chondral bone, and how tissue source and cell culture conditions can affect regenerative potential. 14 In pre-clinical studies, human stem cells have been employed in both immune competent rodents, 2 , 14 , 15 and lagomorphs, 16 , 17 although they have not yet been reported in human clinical trials. 18 However, as has been known for intravenous delivery of MSCs, which become trapped in the lung microvasculature within minutes and cannot be found days later, 19 the dwell time of MSC in OA joints is also thought to be transient.…”

Section: Introductionmentioning

confidence: 99%

Umbilical Cord Stem Cell Lysate: A New Biologic Injectate for the Putative Treatment of Acute Temporomandibular Joint Inflammation

Ward,

Gill,

Liddell

et al. 2023

JIR

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To compare in vivo, the acute anti-inflammatory effects of a lysate derived from human umbilical perivascular mesenchymal cells with the cells themselves in both an established hind-paw model of carrageenan-induced inflammation and also in the inflamed temporomandibular joint. Study Design: Human umbilical cord perivascular cells were harvested and cultured in xeno-and serum-free conditions to P3. In addition, P3 cells were used to prepare a proprietary 0.22 micron filtered lysate. First, CD1 immunocompetent mice underwent unilateral hind-paw injections of carrageenan for induction of inflammation, followed immediately by treatment with saline (negative control), 1% cell lysate, or viable cells. The contralateral paw remained un-injected with carrageenan. Paw circumference was measured prior to injections and 48 hr later and myeloperoxidase and TNF-alpha concentrations were measured post-sacrifice in excised tissue. Second, immunocompetent Male Wistar rats underwent unilateral intra-articular temporomandibular (TMJ) injections from the same treatment groups and were sacrificed at 4 and 48 hr post-injection. The contralateral TMJ remained un-injected with carrageenan. Articular tissue and synovial aspirates, from the treated TMJ were obtained for histologic and leukocyte infiltration analyses. Results:The lysate and cell-treated hind-paw demonstrated reduced tissue edema, and significantly lower concentrations of myeloperoxidase and TNF-alpha at 48 hr compared to untreated controls. Treated TMJs demonstrated lower concentrations of leukocytes in the synovium compared to controls and histologic evidence, in the peri-articular tissue, of reduced inflammation. Conclusion:In this preliminary study, both the human umbilical perivascular cells and a highly diluted lysate produced therefrom were anti-inflammatory.

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