Recently the significance of bile salt hydrolase (BSH, EC 3.1.24) enzyme has been increased since it is highly effective in reducing serum cholesterol levels in humans and animals. High cholesterol level is found as an important reason of atherosclerosis which results in cardiovascular diseases (CVD's). In this study, the search for novel BSH producing bacteria leads to their finding in soil from fish waste-yards. The soil bacteria with potent bile salt hydrolase production and bile tolerant activity was isolated through sequential screening methods and identified based on its phenotypic and genotypic characteristics as Staphylococcus saprophyiticus ZABR2. The media and culture conditions of this selected isolate were optimized for the maximum production of the significant enzyme. The optimum production of the enzyme was observed in sodium glutamate medium, with glucose as carbon source, peptone as nitrogen source, medium pH 5 and when incubated at 45ºC, 160 rpm for 14 hours. A two fold increase in the production of enzyme was observed in the optimized media.
L-Asparaginase is an enzyme (EC3.5.1.1) used as chemotherapeutic agent for the treatment of Acute Lymphoblastic Leukemia (ALL). The commercial L-Asparaginase now available is of bacterial origin and since it shows many side effects, search for alternative sources of this enzyme is highly necessary, owing to its high therapeutic significance. In the present study an attempt has been made to isolate bacterial endophytes, with L-Asparaginase potentiality, from medicinal plants. From 16 different medicinal plants 127 bacterial endophytes have been isolated, among which 51 endophytes were capable of producing L-Asparaginase enzyme. Two isolates that showed excellent enzyme activity as 1.436 U/ml and 1.827 U/ml were identified, based on biochemical tests and 16s rRNA gene sequencing, as Acinetobacter baumannii and Bacillus subtilis which were isolated from the medicinal plants, Annona muricata and Averrhoa carambola respectively. The phylogenetic tree for the two isolates were constructed based on the 16s rRNA blast results and the 16s rRNA gene sequences were deposited in gene bank and accession numbers were received.
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