Isolation screening and identification of bile salt hydrolase producing bacteria from waste samples

Abstract

Recently the significance of bile salt hydrolase (BSH, EC 3.1.24) enzyme has been increased since it is highly effective in reducing serum cholesterol levels in humans and animals. High cholesterol level is found as an important reason of atherosclerosis which results in cardiovascular diseases (CVD's). In this study, the search for novel BSH producing bacteria leads to their finding in soil from fish waste-yards. The soil bacteria with potent bile salt hydrolase production and bile tolerant activity was isolated through sequential screening methods and identified based on its phenotypic and genotypic characteristics as Staphylococcus saprophyiticus ZABR2. The media and culture conditions of this selected isolate were optimized for the maximum production of the significant enzyme. The optimum production of the enzyme was observed in sodium glutamate medium, with glucose as carbon source, peptone as nitrogen source, medium pH 5 and when incubated at 45ºC, 160 rpm for 14 hours. A two fold increase in the production of enzyme was observed in the optimized media.

Isolation and characterization of cellulase producing bacteria from forest, cow dung, Dashen brewery and agro-industrial waste

“…isolated bacteria from specific cellulosic sources have a high potential for producing cellulase enzymes, particularly with increased endo-β-1,4-glucanase activity. The identified isolates were Bacillus species through BLAST comparisons [37], which agrees with our current study.…”