The effects of dose and dose-rate were investigated for single-particle cryo-electron microscopy using stroboscopic data collection. A dose-rate effect was observed favoring lower flux densities.
Background:The nucleosome assembly protein (NAP) family facilitates the dynamic assembly of nucleosomes. Results: We reveal the large molecular assemblies of NAP-histone complexes. Conclusion: NAP-histone complexes assemble to form a heterogeneous population of ring-like scaffolds. Significance: Knowledge of the oligomeric states of NAP-histone complexes is crucial for understanding their diverse functions.
In this paper, we propose an automatic and sequential method for the registration of an image sequence of a road area without ignoring scene-induced motion. This method contributes to a larger work, aiming at vehicle tracking. A typical image sequence is recorded from a helicopter hovering above the freeway. The demand for automation is inevitable due to the large number of images and continuous changes in the traffic situation and weather conditions. A framework is designed and implemented for this purpose. The registration errors are removed in a sequential way based on two homography assumptions. First, an approximate registration is obtained, which is efficiently refined in a second step, using a restricted search area. The results of the stabilization framework are demonstrated on an image sequence consisting of 1500 images and show that our method allows a registration between arbitrary images in the sequence with a geometric error of zero in pixel accuracy.
The typical dose used to record cryo-electron microscopy images from vitrified biological specimens is so high that radiation-induced structural alterations are bound to occur during data acquisition. Integration of all scattered electrons into one image can lead to significant blurring, particularly if the data are collected from an unsupported thin layer of ice suspended over the holes of a support film. Here, the dose has been fractioned and exposure series have been acquired in order to study beam-induced specimen movements under low dose conditions, prior to bubbling. Gold particles were added to the protein sample as fiducial markers. These were automatically localized and tracked throughout the exposure series and showed correlated motions within small patches, with larger amplitudes of motion vectors at the start of a series compared with the end of each series. A non-rigid scheme was used to register all images within each exposure series, using natural neighbor interpolation with the gold particles as anchor points. The procedure increases the contrast and resolution of the examined macromolecules.
Not all video frames are equally informative for recognizing an action. It is computationally infeasible to train deep networks on all video frames when actions develop over hundreds of frames. A common heuristic is uniformly sampling a small number of video frames and using these to recognize the action. Instead, here we propose full video action recognition and consider all video frames. To make this computational tractable, we first cluster all frame activations along the temporal dimension based on their similarity with respect to the classification task, and then temporally aggregate the frames in the clusters into a smaller number of representations. Our method is end-to-end trainable and computationally efficient as it relies on temporally localized clustering in combination with fast Hamming distances in feature space. We evaluate on UCF101, HMDB51, Breakfast, and Something-Something V1 and V2, where we compare favorably to existing heuristic frame sampling methods.
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