The effects of dose and dose-rate were investigated for single-particle cryo-electron microscopy using stroboscopic data collection. A dose-rate effect was observed favoring lower flux densities.

Background:The nucleosome assembly protein (NAP) family facilitates the dynamic assembly of nucleosomes. Results: We reveal the large molecular assemblies of NAP-histone complexes. Conclusion: NAP-histone complexes assemble to form a heterogeneous population of ring-like scaffolds. Significance: Knowledge of the oligomeric states of NAP-histone complexes is crucial for understanding their diverse functions.

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In this paper, we propose an automatic and sequential method for the registration of an image sequence of a road area without ignoring scene-induced motion. This method contributes to a larger work, aiming at vehicle tracking. A typical image sequence is recorded from a helicopter hovering above the freeway. The demand for automation is inevitable due to the large number of images and continuous changes in the traffic situation and weather conditions. A framework is designed and implemented for this purpose. The registration errors are removed in a sequential way based on two homography assumptions. First, an approximate registration is obtained, which is efficiently refined in a second step, using a restricted search area. The results of the stabilization framework are demonstrated on an image sequence consisting of 1500 images and show that our method allows a registration between arbitrary images in the sequence with a geometric error of zero in pixel accuracy.