Coumarins are the most important class of natural compounds found widely in various plants. Many coumarin derivatives with different biological and pharmacological activities have been synthesized. In this study, the antiapoptotic and cytotoxic effects and DNA-binding properties of some synthetic coumarin derivatives (4b, 4d, 4f, 4 g (DBP-g), 4 h and 4j) against K562 cell lines were investigated using different techniques. MTT assay indicated that the DBP-g compound was more active than other derivatives, with a IC 50 value of 55 μM, and therefore this compound was chosen for further investigation. Apoptosis induction was assessed using acridine orange/ethidium bromide double-staining and cell-cycle analysis. In addition, in vitro DNA-binding studies were carried out using ultraviolet-visible light absorption and fluorescence spectroscopy, as well as viscosity measurement and molecular modelling studies. In vitro results indicated that DBP-g interacted with DNA through a groovebinding mode with a binding constant (K b ) of 1.17 × 10 4 M −1 . In agreement with other experimental data, molecular docking studies showed that DBP-g is a minor groove binder. Overall, it can be concluded that DBP-g could be used as an effective and novel chemotherapeutic agent.
The relationship between ABO blood group distribution and Peptic Ulcer Disease (PUD) has been widely evaluated in the past. But data concerning the same evaluation are very limited in Iran. This study sought to determine the distribution of ABO blood group in patients with PUD in Iranian subjects. Eighty-one patients with PUD (51 male and 30 female; mean age: 49 +/- 18 years) who attended our endoscopy section were enrolled. Blood samples were used for ABO/Rhesus (Rh) blood group antigen typing. The ABO blood group phenotype distribution in subjects was as follows: 37.1% (30/81) for group A, 23.4% (19/81) for group B, 35.6% (28/81) for group O and 4.9% (4/81) for group AB. Rh positivity was found in 63% (51/81) of patients. In local healthy population, ABO/Rh blood group distribution was 33.8, 20.7, 34.7, 8.4 and 89.6% for A, B, O, AB and Rh, respectively. AB blood group distribution in healthy population was higher than PUD (8.4 vs 4.9%). In contrast, Rh positivity of PUD in Iran is lower than healthy subjects (63 vs 89.6%). Variation in the results of studies is related to different study communities. According to these results, probably ABO/Rh blood group has an important role in patients with peptic ulceration. The functional significance of ABO blood group distribution might be associated with biological behavior of PUD. The impact of blood group on PUD may be a focus for further studies.
Cardiac syndrome X (CSX) is characterized by chest pain, typical angina pectoris, abnormal exercise test result and normal coronary arteries. Microvascular dysfunction and enhanced oxidative stress are the mechanisms suspected to play an important role in the pathogenesis of CSX. Thus we aimed to evaluate the oxidative stress status of 28 patients with CSX (14 male/14 female, mean age 49.5 ± 9.3 years) and 24 age-and gender-matched healthy controls (10 male/14 female, mean age 45.6 ± 5.7 years). Blood samples were drawn for measurement of malodialdehyde (MDA), as a marker of lipid peroxidation, glutathione (GSH) and superoxide dismutase (SOD) activity, as antioxidant markers and ferric reducing ability of plasma (FRAP), as a marker of total antioxidant capacity. There was significant increase in the levels of MDA in CSX patients comparing to controls (3.8 ± 0.12 vs 3.3 ± 0.14 mM, respectively; p = 0.006). But the levels of FRAP in CSX patients were significantly lower than those controls (504 ± 19 vs 568 ± 26 µM, respectively; p = 0.046). Also, GSH levels and SOD activity in patients were significantly lower than those of the controls (GSH: 133.6 ± 5.4 vs 152.5 ± 7.8 mmol/g Hb, p = 0.048; SOD: 386 ± 34 vs 578 ± 38 U/g Hb, p = 0.0001). It may be concluded that there is systemic oxidative stress in CSX patients. Considerable changes of antioxidant concentrations, indicating a compensatory mechanism to cope with increased oxidative stress in CSX patients and the body's antioxidant defence mechanisms try to minimize oxidative stress damage.
Background Reflux esophagitis (RE) is main manifestation of gastroesophageal reflux disease with esophageal injury. We aimed to evaluate the blood pressure among RE patients after diagnosis compared to non-RE patients.Methods Seventy-four RE patients (39 male/35 female; mean age: 42.2 AE 14.3 years) were enrolled in this study. RE patients were those who had heartburn and/ or regurgitation with endoscopy-positive erosion(s) in the distal esophagus. Seventeen non_RE patients (7 male/10 female; mean age: 40.7 AE 12.1 years) were those who had not history and symptoms of gatroesophageal reflux disease in endoscopy. Their age, body mass index, systolic and diastolic blood pressures were recorded.
ResultsThe levels of systolic blood pressure in RE patients were significantly higher than in those non-ER patients. (87.0 AE 17.9 vs. 75.3 AE 12.8 mmHg, respectively; p ¼ 0.013). Also, there was significant increasing in the levels of diastolic blood pressure between RE and non_RE patients (131.6 AE 20.7 vs. 117.2 AE 20.0, respectively; p ¼ 0.008). There were no significant differences in BMI of RE patients versus non-RE patients (25.7 AE 4.7 vs. 26.0 AE 5.0, respectively, p ¼ 0.806).Conclusions We found an association between experiencing heartburn and/ or acid regurgitation with blood pressure. Confirmation of these findings, and further investigation of the pathophysiological role that biochemical parameters may play in RE, and possibly Barrett's esophagus, are warranted.Objectives The accuracy of noninvasively estimated central systolic blood pressure (SBP) remains controversial. The present study investigated the accuracy of a new oscillometric method by direct comparison with the analysis of the invasive brachial pressure waveform.Methods We enrolled 50 patients (37 men, age range 30-84 years) indicated for cardiac catheterization. Right brachial pressure and central aortic pressure were simultaneously recorded using a dual-sensor pressure catheter, at steady state and 3 minutes after administration of a sublingual nitroglycerin. Noninvasive left arm SBP and diastolic blood pressure (DBP) were also simultaneously measured using an oscillometric blood pressure monitor that was designed to record pulse volume phlethysmography (PVP) immediately after the measurement of noninvasive blood pressures. Central SBP was estimated by the recently proposed comprehensive analysis of the PVP waveform calibrated by the noninvasive SBP and DBP, and also by identification of the late systolic shoulder (SBP2) of the invasive brachial pressure waveform. The reproducibility of the invasive central SBP by the noninvasive and invasive estimation methods was examined using the concordance correlation coefficient.Results Overall, the invasive central aortic SBP ranged 85.6$175.5 with a mean of 123.6 AE 20.5 mmHg. The concordance correlation coefficient for the noninvasively estimated central SBP was 0.93 (95% CI 0.91$0.95), which was significantly better than that for the noninvasive SBP (0.87, 0.83$0.90) and was not significantly different ...
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