Sugar cane processing sites are characterised by high sugar/hemicellulose levels, available moisture and warm conditions, and are relatively unexplored unique microbial environments. The PhyloChip microarray was used to investigate bacterial diversity and community composition in three Australian sugar cane processing plants. These ecosystems were highly complex and dominated by four main Phyla, Firmicutes (the most dominant), followed by Proteobacteria, Bacteroidetes, and Chloroflexi. Significant variation (p < 0.05) in community structure occurred between samples collected from ‘floor dump sediment’, ‘cooling tower water’, and ‘bagasse leachate’. Many bacterial Classes contributed to these differences, however most were of low numerical abundance. Separation in community composition was also linked to Classes of Firmicutes, particularly Bacillales, Lactobacillales and Clostridiales, whose dominance is likely to be linked to their physiology as ‘lactic acid bacteria’, capable of fermenting the sugars present. This process may help displace other bacterial taxa, providing a competitive advantage for Firmicutes bacteria.

Objective The aim of this study was to evaluate the efficacy and safety of transvaginal ultrasound guided aspiration of ovarian endometrioma and ethanol sclerotherapy before controlled ovarian hyperstimulation (COH) and intrauterine insemination (IUI) or assisted reproductive technology (ART) in patients with recurrent ovarian endometrioma. Design Prospective study. Setting Infertility Care and Research Center (ICRC Pvt Ltd.), Dhaka, Bangladesh. Materials and methods Fifty-three patients with infertility and recurrent ovarian endometrioma were the target population of this study. Sclerotherapy was performed under sedation (Pethidine) or G/A (propofol) and transvaginal ultrasound guidance. An 16-guage, double-lumen needle was inserted into the endometrioma and the cyst contents were sequentially aspirated and flushed with sterile saline until the aspirated fluid was clear. Ninety-five percent ethyle alcohol (ethanol) was then instilled into the cyst and kept in situ. Ultrasound was performed 6 weeks, 3, 6 and 12 months later to assess the efficacy of treatment. Controlled ovarian hyperstimulation and IUI or ART was performed according to patients’ profile and desire. Main outcome measures Resolution and recurrence of endometrioma and pregnancy rate. Results Thirty-nine patients had unilateral, 14 had bilateral, 36 had single and 17 had more than one cysts. Size of the cysts were 3.5 to 10 cm, average 6.75 ± 1.64 cm. Complete resolution of cyst took place in 42 (79.25%) cases. Eleven patients had persistence and refilling of cyst. Four of them needed reaspiration. No complications developed in any case. Thirty patients (52 cycles) underwent COH and IUI and 5 (16.57%) of them got pregnant. Forty-one patients underwent ART (63 cycles) and 13 (31.71%) got pregnant. Six (11.32) patients developed small cyst <3 cm within 1 year during the course of treatment. Conclusion Sclerotherapy with 95% ethanol is a simple, effective and safe alternative to surgical intervention for treatment of recurrent ovarian endometriomas before COH and IUI or ART. How to cite this article Begum M R, Ehsan M, Ehsan N, Santa MSB, Khan F, Sharmin F. Sclerotherapy with Ethanol: An Effective and Safe Alternative to Potentially Complex Surgical Treatment of Recurrent Ovarian Endometrioma. J South Asian Feder Obst Gynae 2015;7(2):97-101.

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Abstract:A study was conducted to investigate the effect of dietary moringa leaf meal (MLM) as a natural alternative to antibiotic on the growth performance, meat quality and carcass yield of broiler chicken. A total of 270 day-old broiler chicks were randomly weighed and assigned to six dietary treatments having 45 birds in each. Each dietary treatment had three replicates of 15 birds and was reared in floor pens. Chicks were distributed in 4 different inclusion levels of MLM in diets; 0.5%, 1.0%, 1.5% and 2.0% with 2 control diets negative and positive. The birds were fed the experimental diets and water was provided without restriction throughout the experimental period. The results showed that significant (P<0.05) effect on final body weight and weight gain in dietary supplementation of 1.5% MLM as compared to that of control group. Better feed conversion (1.53) was observed at 1.5% MLM group. Different levels of MLM exhibit significant influence on meat yield performance of broilers. There was no significant difference (P>0.05) observed among the average live weight, thigh meat, wing meat, drumstick meat, gizzard and abdominal fat of broilers. Breast meat, heart, liver, large intestine and dressing percentage showed significant difference. Lower mortality was found on diet supplemented with MLM. It is concluded that 1.5% MLM in broiler diets can be used as natural feed additive for enhancing growth performance, organ development, meat yield and serum cholesterol of broilers as well as replacing of oxytetracycline.

Information on cholesterol intake through restaurant meal is of high concern because of increasing eat-out population. Since nutrient labeling is not mandatory for restaurant food in Korea, cholesterol database on restaurant menu is unavailable. This study was performed to construct regional and national cholesterol database on primary Korean restaurant foods including 30 soup/stew, 24 rice dishes, and 27 noodles. From 2009 to 2012, Korea Ministry of Food and Drug Safety collected total 5832 foods (81 food types ×6 regions ×12 restaurants) nationwide and then 486 composites representing food types and regions were prepared for cholesterol analysis. Cholesterol contents of 486 composite samples were highly affected by recipe, food type, seasonality of ingredients, and geographical location, showing the range of 1.1-143.0, 1.5-85.1, and 0.4-62.2 mg/100 g for soup/stew, rice dishes, and noodles, respectively. The highest cholesterol value was observed in - (spicy fish roe soup) while - (buckwheat noodle in beef stock) showed the lowest among all samples. Most foods contain relatively low cholesterol content, but the serving size and consumption frequency of dishes should be considered in order not to exceed the recommended daily intake limit (300 mg cholesterol). Saponification coupled with gas chromatography applied for cholesterol analysis was reliable based on accuracy (95% > recovery) and precision (repeatability <4% and reproducibility <8%). Quality control chart monitored for 4 years showed that all analyses were under the control. This study provides reliable and representative cholesterol contents of Korean restaurant key foods, which can be utilized for assessments of cholesterol intake in the current Korean diet.