Dengue viruses are the most prevalent arthropod-borne viral diseases in humans, infecting 50-100 million people each year. Its serotypes are the most common causes of arboviral illness, putting half of the world's population at risk of infection. Because there is no vaccine or antiviral medicines, the only way to manage the disease is to reduce the Aedes mosquito vectors. DENV infection can be asymptomatic or cause a self-limiting, acute febrile illness with varying degrees of severity. High fever, headache, stomach discomfort, rash, myalgia, and arthralgia are the typical symptoms of dengue fever (DF). Thrombocytopenia, vascular leakage, and hypotension are symptoms of severe dengue, dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS). Systemic shock characterizes DSS, which can be deadly. Dengue virus infection pathogenesis is linked to a complex interaction between virus, host genes, and host immune response. Major drivers of disease vulnerability include host factors such as antibody-dependent enhancement (ADE), memory cross-reactive T cells, anti-DENV NS1 antibodies, autoimmunity, and genetic variables. The NS1 protein and anti-DENV NS1 antibodies were thought to be involved in the development of severe dengue. The progressive infection may change the cytokine response of cross reactive CD4+ T cells. The need for dengue vaccines that can generate strong protective immunity against all four serotypes is required. To create such vaccines, a thorough understanding of DENV adaptive immunity is required. Structural and functional research have shown that the degree of prM protein cleavage as well as the ensemble of conformational states sampled by virions influence DENV sensitivity to antibody-mediated neutralization, which has crucial implications for vaccine formulation.
Stem cells are undifferentiated, immature, and unspecialized cells having huge potential for differentiation and proliferation into the specialized functionalized cells. More recently, CSC has been described in breast cancer and brain tumors where they make up as few as 1% of the cells in a tumor. The features of cancer stem cells are just like normal stem cells but their replication rate many times faster than normal cells. Regenerative medicines are based on stem cells, are potentially useful to regenerate damaged cells, tissues, organs and replace cancer cells with normal cells. Induced pluripotent stem cells are the most important candidates for regenerative medicines, tissue engineering, cell reprogramming, and 3D printing. Cancer Stem Cells (CSCs) have a tumorinitiating capacity and play crucial roles in tumor metastasis, relapse and chemo/ radioresistance. Because CSCs are resistant to chemotherapeutic drugs and cause recurrence of cancer and also have the ability to be regenerated; they can cause serious problems in the treatment of various cancers. Numerous biocompatible biomaterials, miRNAs, nanomaterial, artificial intelligence, and machine learning are uses to reprograms stem cells into regenerative medicines for the treatment of cancer. The present paper describes the applications and importance of stem cells in regenerative medicines, cancer stem cells targeting therapies, and the role of miRNAs in cancer stem cells targeting.
E. coli and L. monocytogenes are all dairy product related pathogens. The presence of these pathogens can lead to contamination. To increase the shelf life of yogurt we have to monitor the temperature at which it is stored, the change in microbial counts, pH, acidity, sensory attributes and percentage of free whey. Adaptation Test Acid demonstrates that the microorganisms such as Salmonella spp., E. coli, and L. monocytogenes are frequently identified to have a higher survival rate in meals when compared with non-adopted ones. When they are exposed to unfavorable growth circumstances such as severely acidic environments. In this study, we evaluated the survival of wild and adapted L. monocytogenes strains, inoculated at the same concentration around 4 log cfu/g; a slow decrease in the loads was observed until d 28 in unflavored inoculated with the wild strain. Three different experiments are performed on yogurt to evaluate the difference between flavored and unflavored yogurt, shelf life of them at different temperatures and to determine the role of E. coli and Listeria monocytogenes and see what changes it brings to the composition of yogurt. First trial is performed at three different temperatures. These are at 4, 8 and 20°C. Both flavored and unflavored yogurt sample showed low viable counts at 4°C until the end of trial. While performing this trial at 4°C the loads are lower in strawberry yogurts as compared to that of unflavored yogurt because potassium sorbate is present in fruit pure and anti-microbial activity is exerted by that. In second trial, E. coli and Listeria monocytogenes are added to the yogurt sample at two concentrations which 2 and 5 log cfu/g which will show a rapid decrease in acidic conditions in both flavored and unflavored yogurt samples. Listeria monocytogenes is very resistant in this case and presence of it can always be seen until the end period. In the third trial, the adaption of the yogurt sample is monitored after the inoculation of microorganisms in it. Between the wild acid adapted strains of L. monocytogenes no statistically significant difference is detected and that must be because to the quick adaption after the inoculation. Pasteurization is one of the main processes which are used to make the dairy products pathogen free and the basic functioning of it is through temperature and discussed in the paper.
Exploring host-HTLV-1 interactions and the molecular processes underpinning HTLV-1-mediated carcinogenesis is crucial for establishing effective treatments for viral infection and associated leukemia/lymphoma. Several HTLV-1 proteins have been shown to play important roles in the cellular transformation and immortalization of infected T cells. Through interactions with MAVS, STING, and RIP1, the HTLV-1 oncoprotein Tax suppresses the innate IFN response, resulting in the inhibition of TBK1-mediated phosphorylation of IRF3/IRF7. The HTLV-1 protein HBZ affects genomic integrity and inhibits target cell death and autophagy. Furthermore, it has been discovered that HBZ promotes the growth of ATL cells and aids in the evasion of infected cells from immunosurveillance. It currently appears that the efficacy of an individual's cytotoxic T cell (CTL) response to HTLV-1 is the most important single predictor of that person's provirus load, which can differ by more than 10,000-fold amongst HTLV-1-infected persons. We examine recent improvements in our knowledge of the pathophysiology, or underlying processes, of the illness produced by HTLV-1 infection in this article. Furthermore, we explore the future approach for targeting HTLV-1-associated malignancies and anti-HTLV-1 therapies. The pathogenic agent of adult T-cell leukemia/lymphoma (ATL) is human T-cell lymphotropic virus type 1 (HTLV-1). ATL is a fast-developing clonal tumour of CD4+ T cells which are cellular and viral protein interactions and pave the way for the discovery of new classes of cellular modulators, which may induce Tax oncogenesis and its impact on survival signalling pathways such as the NF-B and PI3K-Akt pathways, therapeutic target opportunities for ATL have been presented in two collaborative studies.
In last twenty years DNA, profiling has become an obligatory technology, which has brought scientists towards forensic identification. Thousands of STR markers are present in human genome but only core set of loci are selected for forensic DNA and human identification. STRs have become famous in forensic labs because even low amount and degraded form can be easily typed. STRs are found in prokaryotes and eukaryotes, including humans. They appear scattered almost evenly throughout the human genome, resulting in about 3% of the genome. However, their distribution in chromosomes is not quite uniform. Usually, STRs occur in the noncoding regions, only about 8% are located in the coding regions. In humans, chromosome 19 has the highest density of STRs. On an average, one STR occurs per 2,000 bp in the human genome. On the basis of repeat units, STRs can be differentiated into different types. The repeats of STR markers are highly variable in human population. STR marker evaluation very precisely figures out individual humans at the molecular level even from very small quantities. STR locus in the identification selected is D7S820 from NIST (National Institute of Standards and Technology). Conditions for STR with the changes in temperature, magnesium ion concentration, primer and setting up, PCR of the marker used to carry out. PCR product is inspected of agarose gel. The results showed that the STR locus being investigated is detectable by PCR. PCR showed that the detection of primer and temperature conditions measured by using the fixed amount of magnesium, D7S820 locus bands are weaker than expected. Using a buffer and setting magnesium condition towards changes in primer and temperature, addition of Taq polymerase at a temperature of 94°C, bands become visible desirably.
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